Severe Ankyrin-R deficiency results in impaired surface retention and lysosomal degradation of RhAG in human erythroblasts

Timothy J. Satchwell, Amanda J. Bell, Bethan R. Hawley, Stephanie Pellegrin, Kathryn E. Mordue, Cees Th B M van Deursen, Nicole Heitink-ter Braak, Gerwin Huls, Mathie P G Leers, Eline Overwater, Rienk Y J Tamminga, Bert van der Zwaag, Elisa Fermo, Paola Bianchi, Richard van Wijk, Ashley M. Toye

Research output: Contribution to journalArticle

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Abstract

Ankyrin-R provides a key link between band 3 and the spectrin cytoskeleton that helps to maintain the highly specialized erythrocyte biconcave shape. Ankyrin deficiency results in fragile spherocytic erythrocytes with reduced band 3 and protein 4.2 expression. We use in vitro differentiation of erythroblasts transduced with shRNAs targeting ANK1 to generate erythroblasts and reticulocytes with a novel ankyrin-R ʼnear null’ human phenotype with less than 5% of normal ankyrin expression. Using this model, we demonstrate that absence of ankyrin negatively impacts the reticulocyte expression of a variety of proteins, including band 3, glycophorin A, spectrin, adducin and, more strikingly, protein 4.2, CD44, CD47 and Rh/RhAG. Loss of band 3, which fails to form tetrameric complexes in the absence of ankyrin, alongside GPA, occurs due to reduced retention within the reticulocyte membrane during erythroblast enucleation. However, loss of RhAG is temporally and mechanistically distinct, occurring predominantly as a result of instability at the plasma membrane and lysosomal degradation prior to enucleation. Loss of Rh/RhAG was identified as common to erythrocytes with naturally occurring ankyrin deficiency and demonstrated to occur prior to enucleation in cultures of erythroblasts from a hereditary spherocytosis patient with severe ankyrin deficiency but not in those exhibiting milder reductions in expression. The identification of prominently reduced surface expression of Rh/RhAG in combination with direct evaluation of ankyrin expression using flow cytometry provides an efficient and rapid approach for the categorization of hereditary spherocytosis arising from ankyrin deficiency.

Original languageEnglish
Pages (from-to)1018-1027
Number of pages10
JournalHaematologica
Volume101
Issue number9
DOIs
Publication statusPublished - 2016

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Ankyrins
Erythroblasts
Reticulocytes
Hereditary Spherocytosis
Erythrocyte Anion Exchange Protein 1
Spectrin
Erythrocytes
Glycophorin
Cytoskeleton
Ear
Flow Cytometry
Cell Membrane

ASJC Scopus subject areas

  • Hematology

Cite this

Satchwell, T. J., Bell, A. J., Hawley, B. R., Pellegrin, S., Mordue, K. E., van Deursen, C. T. B. M., ... Toye, A. M. (2016). Severe Ankyrin-R deficiency results in impaired surface retention and lysosomal degradation of RhAG in human erythroblasts. Haematologica, 101(9), 1018-1027. https://doi.org/10.3324/haematol.2016.146209

Severe Ankyrin-R deficiency results in impaired surface retention and lysosomal degradation of RhAG in human erythroblasts. / Satchwell, Timothy J.; Bell, Amanda J.; Hawley, Bethan R.; Pellegrin, Stephanie; Mordue, Kathryn E.; van Deursen, Cees Th B M; Heitink-ter Braak, Nicole; Huls, Gerwin; Leers, Mathie P G; Overwater, Eline; Tamminga, Rienk Y J; van der Zwaag, Bert; Fermo, Elisa; Bianchi, Paola; van Wijk, Richard; Toye, Ashley M.

In: Haematologica, Vol. 101, No. 9, 2016, p. 1018-1027.

Research output: Contribution to journalArticle

Satchwell, TJ, Bell, AJ, Hawley, BR, Pellegrin, S, Mordue, KE, van Deursen, CTBM, Heitink-ter Braak, N, Huls, G, Leers, MPG, Overwater, E, Tamminga, RYJ, van der Zwaag, B, Fermo, E, Bianchi, P, van Wijk, R & Toye, AM 2016, 'Severe Ankyrin-R deficiency results in impaired surface retention and lysosomal degradation of RhAG in human erythroblasts', Haematologica, vol. 101, no. 9, pp. 1018-1027. https://doi.org/10.3324/haematol.2016.146209
Satchwell, Timothy J. ; Bell, Amanda J. ; Hawley, Bethan R. ; Pellegrin, Stephanie ; Mordue, Kathryn E. ; van Deursen, Cees Th B M ; Heitink-ter Braak, Nicole ; Huls, Gerwin ; Leers, Mathie P G ; Overwater, Eline ; Tamminga, Rienk Y J ; van der Zwaag, Bert ; Fermo, Elisa ; Bianchi, Paola ; van Wijk, Richard ; Toye, Ashley M. / Severe Ankyrin-R deficiency results in impaired surface retention and lysosomal degradation of RhAG in human erythroblasts. In: Haematologica. 2016 ; Vol. 101, No. 9. pp. 1018-1027.
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