Severe aplastic anemia (SAA): Response to cyclosporin A (CyA) in vivo and in vitro

J. Tong, A. Bacigalupo, G. Piaggio, O. Figari, M. T. Van Lint, D. Occhini, T. Lamparelli, F. Gualandi, A. M. Marmont

Research output: Contribution to journalArticle

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Abstract

The aim of the present study was to test the effect of cyclosporin A (CyA) in vitro on CFU-GM growth from patients with severe aplastic anemia (SAA). For this purpose, bone marrow (BM) cells from 9 SAA patients and 5 healthy individuals were incubated with or without CyA and then cultured for CFU-GM growth in the presence of exogenous recombinant human GM-CSF (30 ng/ml). SAA patients were tested before or after treatment with CyA, or after treatment with antilymphocyte globulin (ALG). In 3 patients responding to CyA, the addition of CyA in vitro enhanced colony growth from 13 ± 10 to 40 ± 20/105 BM cells (p=0.01) - the median increment of colony formation was 2.4-fold. In 5 ALG responders, CyA produced no increment of CFU-GM growth (from 14 ± 26 to 15 ± 16/105 BM cells, p=0.1). CyA did not enhance significantly CFU-GM growth in normal controls (from 57 ± 45 to 58 ± 81/105 BM cells, p=0.9). In conclusion, it would appear that some patients with SAA can respond to CyA in vivo and in vitro, and ALG responders are not necessarily among these. This is in keeping with different mechanisms of action of CyA and ALG and possibly with the existence of distinct pathogenetic pathways in SAA.

Original languageEnglish
Pages (from-to)212-216
Number of pages5
JournalEuropean Journal of Haematology
Volume46
Issue number4
Publication statusPublished - 1991

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Aplastic Anemia
Cyclosporine
Granulocyte-Macrophage Progenitor Cells
Antilymphocyte Serum
Bone Marrow Cells
Growth
In Vitro Techniques
Granulocyte-Macrophage Colony-Stimulating Factor
Therapeutics

ASJC Scopus subject areas

  • Hematology

Cite this

Tong, J., Bacigalupo, A., Piaggio, G., Figari, O., Van Lint, M. T., Occhini, D., ... Marmont, A. M. (1991). Severe aplastic anemia (SAA): Response to cyclosporin A (CyA) in vivo and in vitro. European Journal of Haematology, 46(4), 212-216.

Severe aplastic anemia (SAA) : Response to cyclosporin A (CyA) in vivo and in vitro. / Tong, J.; Bacigalupo, A.; Piaggio, G.; Figari, O.; Van Lint, M. T.; Occhini, D.; Lamparelli, T.; Gualandi, F.; Marmont, A. M.

In: European Journal of Haematology, Vol. 46, No. 4, 1991, p. 212-216.

Research output: Contribution to journalArticle

Tong, J, Bacigalupo, A, Piaggio, G, Figari, O, Van Lint, MT, Occhini, D, Lamparelli, T, Gualandi, F & Marmont, AM 1991, 'Severe aplastic anemia (SAA): Response to cyclosporin A (CyA) in vivo and in vitro', European Journal of Haematology, vol. 46, no. 4, pp. 212-216.
Tong, J. ; Bacigalupo, A. ; Piaggio, G. ; Figari, O. ; Van Lint, M. T. ; Occhini, D. ; Lamparelli, T. ; Gualandi, F. ; Marmont, A. M. / Severe aplastic anemia (SAA) : Response to cyclosporin A (CyA) in vivo and in vitro. In: European Journal of Haematology. 1991 ; Vol. 46, No. 4. pp. 212-216.
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