Sex-, tissue-, and stage-specific expression of a vitelline membrane protein gene from region 32 of the second chromosome of Drosophila melanogaster

S. Gigliotti, F. Graziani, L. De Ponti, F. Rafti, A. Manzi, G. Lavorgna, G. Gargiulo, C. Malva

Research output: Contribution to journalArticlepeer-review

Abstract

This study isolated cDNA clones from egg-chamber and adult female Drosophila cDNA libraries using as probe a DNA fragment from a 200-kb 'chromosome walk' in region 32E of the second chromosome of D. melanogaster. The present authors believe that these clones correspond to a new vitelline membrane protein (VMP) gene because 1) cDNA clones in Northern blots identify a transcript expressed in a tissue- and stage-specific manner: stage 10 eggchambers; 2) the sequence of cDNAs and of the genomic subclone shows homology with the other VMP genes that have been identified to date; 3) the amino acid composition of the translational product has the high content of proline and alanine characteristic of VMPs. Two aspects emerging from this study are worth stressing: 1) the presence of a hydrophobic domain that is highly conserved in all the VMP genes; and 2) the particularly narrow period of expression of the isolated gene, which could be related to the mechanism of vitelline membrane assembly.

Original languageEnglish
Pages (from-to)33-41
Number of pages9
JournalDevelopmental Genetics
Volume10
Issue number1
Publication statusPublished - 1989

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Genetics

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