Sgk1 enhances RANBP1 transcript levels and decreases taxol sensitivity in RKO colon carcinoma cells

R. Amato, D. Scumaci, L. D'antona, R. Iuliano, M. Menniti, M. Di Sanzo, M. C. Faniello, E. Colao, P. Malatesta, A. Zingone, V. Agosti, F. S. Costanzo, A. M. Mileo, M. G. Paggi, F. Lang, G. Cuda, P. Lavia, N. Perrotti

Research output: Contribution to journalArticlepeer-review

Abstract

The serum- and glucocorticoid-regulated kinase (Sgk1) is essential for hormonal regulation of epithelial sodium channel-mediated sodium transport and is involved in the transduction of growth factor-dependent cell survival and proliferation signals. Growing evidence now points to Sgk1 as a key element in the development and/or progression of human cancer. To gain insight into the mechanisms through which Sgk1 regulates cell proliferation, we adopted a proteomic approach to identify up- or downregulated proteins after Sgk1-specific RNA silencing. Among several proteins, the abundance of which was found to be up- or downregulated upon Sgk1 silencing, we focused our attention of RAN-binding protein 1 (RANBP1), a major effector of the GTPase RAN. We report that Sgk1-dependent regulation of RANBP1 has functional consequences on both mitotic microtubule activity and taxol sensitivity of cancer cells.

Original languageEnglish
Pages (from-to)4572-4578
Number of pages7
JournalOncogene
Volume32
Issue number38
DOIs
Publication statusPublished - Sep 19 2013

Keywords

  • mitotic microtubule stabilization
  • RANBP1
  • Sgk1
  • taxol sensitivity

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

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