Shc proteins are phosphorylated and regulated by the v-Src and v-Fps protein-tyrosine kinases

J. McGlade, A. Cheng, G. Pelicci, P. G. Pelicci, T. Pawson

Research output: Contribution to journalArticlepeer-review

Abstract

The mammalian shc gene encodes two overlapping proteins of 46 and 52 kDa, each with a C-terminal Src homology 2 (SH2) domain and an N-terminal glycine/proline-rich sequence, that induce malignant transformation when overexpressed in mouse fibroblasts. p46(shc), p52(shc), and an additional 66- kDa shc gene product become highly tyrosine phosphorylated in Rat-2 cells transformed by the v-src or v-fps oncogene. Experiments using temperature- sensitive v-src and v-fps mutants indicate that Shc tyrosine phosphorylation is rapidly induced upon activation of the v-Src or v-Fps tyrosine kinases. These results suggest that Shc proteins may be directly phosphorylated by the v-Src and v-Fps oncoproteins in vivo. In cells transformed by v-src or v-fps, or in normal cells stimulated with epidermal growth factor, Shc proteins complex with a poorly phosphorylated 23-kDa polypeptide (p23). Activated tyrosine kinases therefore regulate the association of Shc proteins with p23 and may thereby control the stimulation of an Shc-mediated signal transduction pathway. The efficient phosphorylation of Shc proteins and the apparent induction of their p23-binding activity in v-src- and v-fps- transformed cells are consistent with the proposition that the SH2-containing Shc polypeptides are biologically relevant substrates of the oncogenic v-Src and v-Fps tyrosine kinases.

Original languageEnglish
Pages (from-to)8869-8873
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number19
DOIs
Publication statusPublished - 1992

Keywords

  • Src homology 2 domain
  • transformation
  • tyrosine phosphorylation

ASJC Scopus subject areas

  • General
  • Genetics

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