Shear stress and VEGF enhance endothelial differentiation of human adipose-derived stem cells

Francesca Colazzo, Fahad Alrashed, Padmini Saratchandra, Ivan Carubelli, Adrian H. Chester, Magdi H. Yacoub, Patricia M. Taylor, Pamela Somers

Research output: Contribution to journalArticle

Abstract

Herein we combine chemical and mechanical stimulation to investigate the effects of vascular endothelial growth factor (VEGF) and physiological shear stress in promoting the differentiation human adipose derived stem cells (ADSCs) into endothelial cells. ADSCs were isolated and characterized; endothelial differentiation was promoted by culturing confluent cells in 50 ng/ml VEGF under physiological shear stress for up to 14 days. Afterwards, endothelial cells were seeded onto collagen or acellular aortic valve matrices and exposed to four culture conditions: shear stress + VEGF; shear stress - VEGF; static + VEGF and static - VEGF. After 7 days, phenotype was investigated. ADSCs subjected to shear stress and VEGF express a comprehensive range of specific endothelial markers (vWF, eNOS and FLT-1 after 7 days and CD31, FLk-1 and VE-cadherin after 14 days) and maintain the phenotype when seeded onto scaffolds. Our protocol proved to be an efficient source of endothelial-like cells for tissue engineering based on autologous ADSC.

Original languageEnglish
Pages (from-to)139-149
Number of pages11
JournalGrowth Factors
Volume32
Issue number5
DOIs
Publication statusPublished - Oct 1 2014

Keywords

  • Adipose-derived stem cells
  • Collagen scaffold
  • differentiation
  • shear stress
  • vascular endothelial growth factor

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Endocrinology
  • Cell Biology
  • Medicine(all)

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  • Cite this

    Colazzo, F., Alrashed, F., Saratchandra, P., Carubelli, I., Chester, A. H., Yacoub, M. H., Taylor, P. M., & Somers, P. (2014). Shear stress and VEGF enhance endothelial differentiation of human adipose-derived stem cells. Growth Factors, 32(5), 139-149. https://doi.org/10.3109/08977194.2014.945642