Shedding of membrane vesicles by tumor and endothelial cells

V. Dolo, S. D'Ascenzo, I. Giusti, D. Millimaggi, G. Taraboletti, A. Pavan

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Shedding of membrane vesicles is a vital phenomenon frequently observed in tumor cells and suggested to be involved in several aspects of tumor progression. Our previous studies have shown that human breast tumor cells rapidly shed membrane vesicles containing matrix metalloproteinases (MMPs). In this study we present that human umbilical vein endothelial cells (HUVEC) as well as different tumor cell lines (human ovarian cancer, CABA I and A2780, and hepatocarcinoma cell line, SK-Hep 1) shed vesicles in the extracellular medium. These vesicles carry MMPs and their inhibitors TIMPs. We conclude that tumor and endothelial cells shed MMP-containing vesicles and this may represent a mechanism for regulating focalized proteolytic activity and a way to interact with microenvironment during tumor angiogenesis.

Original languageEnglish
Pages (from-to)127-133
Number of pages7
JournalItalian journal of anatomy and embryology = Archivio italiano di anatomia ed embriologia
Volume110
Issue number2 SUPPL. 1
Publication statusPublished - 2005

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Endothelial Cells
Matrix Metalloproteinases
Membranes
Neoplasms
Tumor Microenvironment
Matrix Metalloproteinase Inhibitors
Human Umbilical Vein Endothelial Cells
Tumor Cell Line
Ovarian Neoplasms
Breast Neoplasms
Cell Line
Extracellular Vesicles

Keywords

  • Angiogenesis
  • Endothelial cells
  • Membrane vesicles
  • Shedding
  • Tumor

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Shedding of membrane vesicles by tumor and endothelial cells. / Dolo, V.; D'Ascenzo, S.; Giusti, I.; Millimaggi, D.; Taraboletti, G.; Pavan, A.

In: Italian journal of anatomy and embryology = Archivio italiano di anatomia ed embriologia, Vol. 110, No. 2 SUPPL. 1, 2005, p. 127-133.

Research output: Contribution to journalArticle

Dolo, V. ; D'Ascenzo, S. ; Giusti, I. ; Millimaggi, D. ; Taraboletti, G. ; Pavan, A. / Shedding of membrane vesicles by tumor and endothelial cells. In: Italian journal of anatomy and embryology = Archivio italiano di anatomia ed embriologia. 2005 ; Vol. 110, No. 2 SUPPL. 1. pp. 127-133.
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