Shedding of the 67-kD laminin receptor by human cancer cells

Maria Karpatová, Elda Tagliabue, Vincent Castronovo, Alessandra Magnifico, Elena Ardini, Daniele Morelli, Dorina Belotti, Maria I. Colnaghi, Sylvie Ménard

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The 67-kD laminin receptor (67LR) is a cell membrane-associated molecule exhibiting high affinity for the basement membrane glycoprotein, laminin. While export of the 67LR toward the extracellular matrix has been recently suggested by electron microscopy studies, there is to date no evidence of shedding of the 67LR from cells. Using two monoclonal antibodies directed against the 67LR, we developed a double-determinant radioimmunoassay that demonstrates that the 67LR is released from cancer cells into the culture medium. The shed molecule exhibited the same apparent molecular weight as that of the membrane-associated 67LR, suggesting that no proteolytic cleavage is involved in the process. Furthermore, we demonstrate that the 67LR is not anchored to the membrane through a glycolsyl-phosphatidylinositol bridge. However, the observation that lactose increased the release of 67LR suggests that a lectin-type interaction is involved in the cell membrane association of this laminin binding protein and the cell surface. Interestingly, the released 67LR recovered after HPLC gel filtration was found free as well as associated to high molecular weight complexes. The free 67LR retained its ability to bind to the cell surface. Our study is the first demonstration that the 67LR is effectively shed by cancer cells. The released free 67LR could play an important role in modulating interactions between cancer cells and laminin during tumor invasion and metastasis.

Original languageEnglish
Pages (from-to)226-234
Number of pages9
JournalJournal of Cellular Biochemistry
Volume60
Issue number2
DOIs
Publication statusPublished - 1996

Fingerprint

Laminin Receptors
Cells
Neoplasms
Laminin
Cell membranes
Molecular Weight
Molecular weight
Cell Membrane
Membranes
Molecules
Membrane Glycoproteins
Lactose
Phosphatidylinositols
Lectins
Basement Membrane
Electron microscopy
Radioimmunoassay
Gel Chromatography
Extracellular Matrix
Culture Media

Keywords

  • adhesion
  • double determinant assay
  • metastasis
  • Monomeric laminin receptor
  • prognostic factor
  • shedding

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

Shedding of the 67-kD laminin receptor by human cancer cells. / Karpatová, Maria; Tagliabue, Elda; Castronovo, Vincent; Magnifico, Alessandra; Ardini, Elena; Morelli, Daniele; Belotti, Dorina; Colnaghi, Maria I.; Ménard, Sylvie.

In: Journal of Cellular Biochemistry, Vol. 60, No. 2, 1996, p. 226-234.

Research output: Contribution to journalArticle

Karpatová, Maria ; Tagliabue, Elda ; Castronovo, Vincent ; Magnifico, Alessandra ; Ardini, Elena ; Morelli, Daniele ; Belotti, Dorina ; Colnaghi, Maria I. ; Ménard, Sylvie. / Shedding of the 67-kD laminin receptor by human cancer cells. In: Journal of Cellular Biochemistry. 1996 ; Vol. 60, No. 2. pp. 226-234.
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