Short and long-term variations in serum calciotropic hormones after a single very large dose of ergocalciferol (vitamin D2) or cholecalciferol (vitamin D3) in the elderly

Elisabetta Romagnoli, Maria Lucia Mascia, Cristiana Cipriani, Valeria Fassino, Franco Mazzei, Emilio D'Erasmo, Vincenzo Carnevale, Alfredo Scillitani, Salvatore Minisola

Research output: Contribution to journalArticle


Context: In humans, few studies have compared the potencies of ergocalciferol and cholecalciferol in improving and maintaining vitamin D status. Objective: Our objective was to evaluate the effects of a single very large dose of both calciferols on serum changes of 25-hydroxyvitamin D [25(OH)D], 1,25-dihydroxyvitamin D [1,25(OH)2D], ionized calcium, and parathyroid hormone (PTH) at baseline, and at 3, 7, 30, and 60 d. Design: This was a prospective randomized intervention study. Setting: The study was performed in a nursing home residence. Participants: A total of 32 elderly female patients (age range 66-97 yr), with vitamin D deficiency was included in the study. Intervention: Participants were randomized into four groups of eight to receive a single dose of 300,000 IU ergocalciferol or cholecalciferol by oral (os) or im route. Results: 25(OH)D levels sharply increased at d 3 only when vitamins were given os. The 30-d basal difference in serum 25(OH)D was significantly greater after cholecalciferol os administration (47.8 ± 7.3 ng/ml) compared with other forms (D3 im: 15.9 ± 11.3; D 2 os: 17.3 ± 4.7; D2 im: 5 ± 4.4; all P <0.001). The area under the curve (AUC) of the serum 25(OH)D against time (AUC60) was: D3 os, 3193 ± 759 ng × d/ml vs. D2 os, 1820 ± 512, P <0.001; and D3 im, 1361 ± 492 vs. D2 im, 728 ± 195, P <0.01. 25(OH)D significantly influences PTH levels at 3 (P <0.03), 7 (P <0.01), 30 (P <0.01), and 60 d (P <0.05). At 60 d, the form of vitamin (cholecalciferol) significantly lowers PTH levels (P = 0.037). Conclusions: Cholecalciferol is almost twice as potent as ergocalciferol in increasing serum 25(OH)D, when administered either by mouth or im. 25(OH)D plays a role in modulating serum PTH.

Original languageEnglish
Pages (from-to)3015-3020
Number of pages6
JournalJournal of Clinical Endocrinology and Metabolism
Issue number8
Publication statusPublished - Aug 2008


ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this