Short-term abstinence from cocaine self-administration, but not passive cocaine infusion, elevates αcaMKII autophosphorylation in the rat nucleus accumbens and medial prefrontal cortex

Lucia Caffino, Chiara Cassina, Giuseppe Giannotti, Alessandro Orrù, Federico Moro, Angelo Di Clemente, Giorgio Racagni, Fabio Fumagalli, Luigi Cervo

Research output: Contribution to journalArticlepeer-review

Abstract

Increases in alpha calcium/calmodulin-dependent protein kinase type II (αCaMKII) activity in the nucleus accumbens shell has been proposed as a core component in the motivation to self-administer cocaine and in priming-induced drug-seeking. Since cocaine withdrawal promotes drug-seeking, we hypothesized that abstinence from cocaine self-administration should enhance αCaMKII as well. We found that short-term abstinence from contingent, but not non-contingent, cocaine i.v. self-administration (2Â h/d for 14Â d; 0.25Â mg/0.1Â ml, 6s infusion) elevates αCaMKII autophosphorylation, but not the kinase expression, in a dynamic, time-and brain region-dependent manner. Increased αCaMKII autophosphorylation in the nucleus accumbens (NAc) and medial prefrontal cortex (mPFC), but not dorsolateral striatum (dlS), was found 24Â h, but not immediately, after the last cocaine self-administration session. Notably, in the mPFC, but not NAc and dlS, αCaMKII autophosphorylation was still enhanced 7Â d later. The persistent enhancement in the mPFC of abstinent rats may represent a previously unappreciated contribution to initial incubation of cocaine-seeking.

Original languageEnglish
Pages (from-to)323-329
Number of pages7
JournalInternational Journal of Neuropsychopharmacology
Volume17
Issue number2
DOIs
Publication statusPublished - Feb 2014

Keywords

  • CaMKII
  • cocaine
  • glutamate
  • medial prefrontal cortex
  • nucleus accumbens
  • yoked control-operant paradigm

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Pharmacology
  • Psychiatry and Mental health

Fingerprint Dive into the research topics of 'Short-term abstinence from cocaine self-administration, but not passive cocaine infusion, elevates αcaMKII autophosphorylation in the rat nucleus accumbens and medial prefrontal cortex'. Together they form a unique fingerprint.

Cite this