Short-time survival and engraftment of bone marrow stromal cells in an ectopic model of bone regeneration

Paolo Giannoni, Silvia Scaglione, Antonio Daga, Cristina Ilengo, Michele Cilli, Rodolfo Quarto

Research output: Contribution to journalArticle

Abstract

In tissue-engineered applications bone marrow stromal cells (BMSCs) are combined with scaffolds to target bone regeneration; animal models have been devised and the cells' long-term engraftment has been widely studied. However, in regenerated bone, the cell number is severely reduced with respect to the initially seeded BMSCs. This reflects the natural low cellularity of bone but underlines the selectivity of the differentiation processes. In this respect, we evaluated the short-term survival of BMSCs, transduced with the luciferase gene, after implantation of cell-seeded scaffolds in a nude mouse model. Cell proliferation/survival was assessed by bioluminescence imaging: light production was decreased by 30% on the first day, reaching a 50% loss within 48h. Less than 5% of the initial signal remained after 2 months in vivo. Apoptotic BMSCs were detected within the first 2 days of implantation. Interestingly, the initial frequency of clonogenic progenitors matched the percentage of in vivo surviving cells. Cytokines and inflammation may contribute to the apoptotic onset at the implant milieu. However, preculturing cells with tumor necrosis factor alpha enhanced survival, allowing detection of 8.1% of the seeded BMSCs 2 months after implantation. Thus culturing conditions may reduce the apoptotic overload of seeded osteoprogenitors, strengthening the constructs' osteogenic potential.

Original languageEnglish
Pages (from-to)489-499
Number of pages11
JournalTissue Engineering - Part A
Volume16
Issue number2
DOIs
Publication statusPublished - Feb 1 2010

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Bone Regeneration
Mesenchymal Stromal Cells
Bone
Scaffolds (biology)
Bone and Bones
Luciferases
Nude Mice
Bioluminescence
Cell Survival
Animal Models
Tumor Necrosis Factor-alpha
Cell Count
Cell Proliferation
Cytokines
Cell proliferation
Inflammation
Light
Animals
Genes
Tissue

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Biomedical Engineering
  • Biomaterials
  • Medicine(all)

Cite this

Short-time survival and engraftment of bone marrow stromal cells in an ectopic model of bone regeneration. / Giannoni, Paolo; Scaglione, Silvia; Daga, Antonio; Ilengo, Cristina; Cilli, Michele; Quarto, Rodolfo.

In: Tissue Engineering - Part A, Vol. 16, No. 2, 01.02.2010, p. 489-499.

Research output: Contribution to journalArticle

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