TY - JOUR
T1 - Shp2 in PC12 cells
T2 - NGF versus EGF signalling
AU - D'Alessio, Amelia
AU - Cerchia, Laura
AU - Amelio, Ivano
AU - Incoronato, Mariarosaria
AU - Condorelli, Gerolama
AU - de Franciscis, Vittorio
PY - 2007/6
Y1 - 2007/6
N2 - The balance between specific signals from different growth factors dictates the biological response of mammalian cells including cell proliferation, differentiation and survival. PC12 cells represent a model of choice to compare the signalling of differentiative growth factors, as NGF, and of mitogenic growth factors, as EGF. In these cells the prolonged activity of the ERK kinase dictates the decision of cells to differentiate. Here we focused on the cytosolic tyrosine phosphatase Shp2 as an established regulator of the Ras-ERK cascade, to elucidate its involvement in determining the stimulation-dependent PC12 cell fate. To this end, we generated PC12 derived cell lines that express the interfering mutant of Shp2 under a tetracycline-inducible promoter. Our findings show that Shp2 participates to the opposite effects induced in PC12 cells by EGF and NGF and that the interactions with the multidocking Gab2 protein mediate such effects.
AB - The balance between specific signals from different growth factors dictates the biological response of mammalian cells including cell proliferation, differentiation and survival. PC12 cells represent a model of choice to compare the signalling of differentiative growth factors, as NGF, and of mitogenic growth factors, as EGF. In these cells the prolonged activity of the ERK kinase dictates the decision of cells to differentiate. Here we focused on the cytosolic tyrosine phosphatase Shp2 as an established regulator of the Ras-ERK cascade, to elucidate its involvement in determining the stimulation-dependent PC12 cell fate. To this end, we generated PC12 derived cell lines that express the interfering mutant of Shp2 under a tetracycline-inducible promoter. Our findings show that Shp2 participates to the opposite effects induced in PC12 cells by EGF and NGF and that the interactions with the multidocking Gab2 protein mediate such effects.
KW - EGF
KW - Neurotrophin signalling
KW - NGF
KW - PC12
KW - Shp2
UR - http://www.scopus.com/inward/record.url?scp=34147175535&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34147175535&partnerID=8YFLogxK
U2 - 10.1016/j.cellsig.2006.12.011
DO - 10.1016/j.cellsig.2006.12.011
M3 - Article
C2 - 17287109
AN - SCOPUS:34147175535
VL - 19
SP - 1193
EP - 1200
JO - Cellular Signalling
JF - Cellular Signalling
SN - 0898-6568
IS - 6
ER -