SI113, a specific inhibitor of the Sgk1 kinase activity that counteracts cancer cell proliferation

Lucia D'Antona, Rosario Amato, Cristina Talarico, Francesco Ortuso, Miranda Menniti, Vincenzo Dattilo, Rodolfo Iuliano, Francesco Gigliotti, Anna Artese, Giosuè Costa, Silvia Schenone, Francesca Musumeci, Claudia Abbruzzese, Lorenzo Botta, Francesco Trapasso, Stefano Alcaro, Marco G. Paggi, Nicola Perrotti

Research output: Contribution to journalArticlepeer-review

Abstract

Background/Aims: Published observations on serum and glucocorticoid regulated kinase 1 (Sgk1) knockout murine models and Sgk1-specific RNA silencing in the RKO human colon carcinoma cell line point to this kinase as a central player in colon carcinogenesis and in resistance to taxanes. Methods: By in vitro kinase activity inhibition assays, cell cycle and viability analysis in human cancer model systems, we describe the biologic effects of a recently identified kinase inhibitor, SI113, characterized by a substituted pyrazolo[3,4-d]pyrimidine scaffold, that shows specificity for Sgk1. Results: SI113 was able to inhibit in vitro cell growth in cancer cells derived from tumors with different origins. In RKO cells, this kinase inhibitor blocked insulin-dependent phosphorylation of the Sgk1 substrate Mdm2, the main regulator of p53 protein stability, and induced necrosis and apoptosis when used as a single agent. Finally, SI113 potentiated the effects of paclitaxel on cell viability. Conclusion: Since SI113 appears to be effective in inducing cell death in RKO cells, potentiating paclitaxel sensitivity, we believe that this new molecule could be efficiently employed, alone or in combination with paclitaxel, in colon cancer chemotherapy.

Original languageEnglish
Pages (from-to)2006-2018
Number of pages13
JournalCellular Physiology and Biochemistry
Volume35
Issue number5
DOIs
Publication statusPublished - May 6 2015

Keywords

  • Apoptosis
  • Cancer
  • Kinase inhibitor
  • Necrosis
  • SGK1
  • Small molecule

ASJC Scopus subject areas

  • Physiology
  • Medicine(all)

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