SIAH-1 interacts with α-tubulin and degrades the kinesin Kid by the proteasome pathway during mitosis

Antonia Germani, Heriberto Bruzzoni-Giovanelli, Arlette Fellous, Sylvie Gisselbrecht, Nadine Varin-Blank, Fabien Calvo

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

SIAH-1, a human homologue of the Drosophila seven in absentia (Sina), has been implicated in ubiquitin-mediated proteolysis of different target proteins through its N-terminal RING finger domain. SIAH-1 is also induced during p53-mediated apoptosis. Furthermore, SIAH-l-transfected breast cancer cell line MCF-7 exhibits an altered mitotic process resulting in multinucleated giant cells. Now, using the two-hybrid system, we identified two new SIAH interacting proteins: Kid (kinesin like DNA binding protein) and α-tubulin. We demonstrate that SIAH is involved in the degradation of Kid via the ubiquitin-proteasome pathway. Our results suggest that SIAH-1 but not its N-terminal deletion mutant, affects the mitosis by an enhanced reduction of kinesin levels. Our results imply, for the first time, SIAH-1 in regulating the degradation of proteins directly implicated in the mitotic process.

Original languageEnglish
Pages (from-to)5997-6006
Number of pages10
JournalOncogene
Volume19
Issue number52
DOIs
Publication statusPublished - Dec 7 2000

Fingerprint

Kinesin
Proteasome Endopeptidase Complex
Tubulin
Ubiquitin
Mitosis
Proteolysis
RING Finger Domains
DNA-Binding Proteins
Giant Cells
Drosophila
Proteins
Apoptosis
Breast Neoplasms
Cell Line

Keywords

  • Kid
  • Kinesin
  • Mitosis
  • SIAH-1
  • Ubiquitin degradation

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Germani, A., Bruzzoni-Giovanelli, H., Fellous, A., Gisselbrecht, S., Varin-Blank, N., & Calvo, F. (2000). SIAH-1 interacts with α-tubulin and degrades the kinesin Kid by the proteasome pathway during mitosis. Oncogene, 19(52), 5997-6006. https://doi.org/10.1038/sj.onc.1204002

SIAH-1 interacts with α-tubulin and degrades the kinesin Kid by the proteasome pathway during mitosis. / Germani, Antonia; Bruzzoni-Giovanelli, Heriberto; Fellous, Arlette; Gisselbrecht, Sylvie; Varin-Blank, Nadine; Calvo, Fabien.

In: Oncogene, Vol. 19, No. 52, 07.12.2000, p. 5997-6006.

Research output: Contribution to journalArticle

Germani, A, Bruzzoni-Giovanelli, H, Fellous, A, Gisselbrecht, S, Varin-Blank, N & Calvo, F 2000, 'SIAH-1 interacts with α-tubulin and degrades the kinesin Kid by the proteasome pathway during mitosis', Oncogene, vol. 19, no. 52, pp. 5997-6006. https://doi.org/10.1038/sj.onc.1204002
Germani A, Bruzzoni-Giovanelli H, Fellous A, Gisselbrecht S, Varin-Blank N, Calvo F. SIAH-1 interacts with α-tubulin and degrades the kinesin Kid by the proteasome pathway during mitosis. Oncogene. 2000 Dec 7;19(52):5997-6006. https://doi.org/10.1038/sj.onc.1204002
Germani, Antonia ; Bruzzoni-Giovanelli, Heriberto ; Fellous, Arlette ; Gisselbrecht, Sylvie ; Varin-Blank, Nadine ; Calvo, Fabien. / SIAH-1 interacts with α-tubulin and degrades the kinesin Kid by the proteasome pathway during mitosis. In: Oncogene. 2000 ; Vol. 19, No. 52. pp. 5997-6006.
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