Sidestream dark field videomicroscopy for in vivo evaluation of vascularization and perfusion of mammary tumours in HER2/neu transgenic mice

Elisa Damiani, Elisa Pierpaoli, Fiorenza Orlando, Abele Donati, Mauro Provinciali

Research output: Contribution to journalArticlepeer-review

Abstract

Angiogenesis plays a key role in tumour growth and the formation of metastases. Angiogenesis inhibitors and antivascular agents may prove useful in the treatment of breast cancer. A comprehensive characterization of the vasculature and perfusion of mammary tumours is a prerequisite for developing new specific drugs. We used sidestream dark field videomicroscopy to evaluate in vivo the vascular network of spontaneous mammary tumours in HER2/neu transgenic mice. The tumour masses showed higher vessel density compared with the healthy mammary gland (median (interquartile range) total vessel density 16.8 (13.4-20.5) vs 9.1 (8.1-10.9) mm/mm2, respectively; P 2. Sidestream dark field imaging is a versatile technique that may be useful for understanding the role of angiogenesis in the progression of breast cancer and its relationship with outcome. It may represent a valuable tool for dynamic monitoring of the effects of new anti-angiogenesis therapies.

Original languageEnglish
Pages (from-to)225-229
Number of pages5
JournalClinical and Experimental Pharmacology and Physiology
Volume42
Issue number2
DOIs
Publication statusPublished - Feb 1 2015

Keywords

  • Angiogenesis
  • Breast cancer
  • Sidestream dark field videomicroscopy

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)
  • Pharmacology
  • Medicine(all)

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