Sieve-based device for MALDI sample preparation. I. Influence of sample deposition conditions in oligonucleotide analysis to achieve significant increases in both sensitivity and resolution

Laura Molin, Simone Cristoni, Sara Crotti, Luigi Rossi Bernardi, Roberta Seraglia, Pietro Traldi

Research output: Contribution to journalArticle

Abstract

Spraying of oligonucleotide-matrix solutions through a stainless steel (ss) sieve (38 μm, 450 mesh) leads to the formation, on the matrix-assisted laser desorption/ionization (MALDI) sample holder, of uniformly distributed microcrystals, well separated from each other. When the resulting sample holder surface is irradiated by laser, abundant molecular species form, with a clear increase in both intensity and resolution with respect to values obtained by 'Dried Droplet', 'Double Layer', and 'Sandwich' deposition methods. In addition, unlike the usual situation, the sample is perfectly homogeneous, and identical spectra are obtained by irradiating different areas. On one hand, the data indicate that this method is highly effective for oligonucleotide MALDI analysis, and on the other, that it can be validly employed for fully automated MALDI procedures.

Original languageEnglish
Pages (from-to)1512-1520
Number of pages9
JournalJournal of Mass Spectrometry
Volume43
Issue number11
DOIs
Publication statusPublished - Nov 2008

Fingerprint

sieves
Sieves
oligonucleotides
Oligonucleotides
Ionization
Desorption
Lasers
desorption
ionization
Equipment and Supplies
preparation
sensitivity
matrices
holders
lasers
Microcrystals
Stainless Steel
microcrystals
spraying
Spraying

Keywords

  • MALDI sample preparation
  • MALDI-MS
  • Oligonucleotides
  • Sample microspots
  • Sieve-based device

ASJC Scopus subject areas

  • Spectroscopy

Cite this

Sieve-based device for MALDI sample preparation. I. Influence of sample deposition conditions in oligonucleotide analysis to achieve significant increases in both sensitivity and resolution. / Molin, Laura; Cristoni, Simone; Crotti, Sara; Bernardi, Luigi Rossi; Seraglia, Roberta; Traldi, Pietro.

In: Journal of Mass Spectrometry, Vol. 43, No. 11, 11.2008, p. 1512-1520.

Research output: Contribution to journalArticle

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AU - Seraglia, Roberta

AU - Traldi, Pietro

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