Abstract
Spraying of oligonucleotide-matrix solutions through a stainless steel (ss) sieve (38 μm, 450 mesh) leads to the formation, on the matrix-assisted laser desorption/ionization (MALDI) sample holder, of uniformly distributed microcrystals, well separated from each other. When the resulting sample holder surface is irradiated by laser, abundant molecular species form, with a clear increase in both intensity and resolution with respect to values obtained by 'Dried Droplet', 'Double Layer', and 'Sandwich' deposition methods. In addition, unlike the usual situation, the sample is perfectly homogeneous, and identical spectra are obtained by irradiating different areas. On one hand, the data indicate that this method is highly effective for oligonucleotide MALDI analysis, and on the other, that it can be validly employed for fully automated MALDI procedures.
| Original language | English |
|---|---|
| Pages (from-to) | 1512-1520 |
| Number of pages | 9 |
| Journal | Journal of Mass Spectrometry |
| Volume | 43 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - Nov 2008 |
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Keywords
- MALDI sample preparation
- MALDI-MS
- Oligonucleotides
- Sample microspots
- Sieve-based device
ASJC Scopus subject areas
- Spectroscopy
Cite this
Sieve-based device for MALDI sample preparation. I. Influence of sample deposition conditions in oligonucleotide analysis to achieve significant increases in both sensitivity and resolution. / Molin, Laura; Cristoni, Simone; Crotti, Sara; Bernardi, Luigi Rossi; Seraglia, Roberta; Traldi, Pietro.
In: Journal of Mass Spectrometry, Vol. 43, No. 11, 11.2008, p. 1512-1520.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Sieve-based device for MALDI sample preparation. I. Influence of sample deposition conditions in oligonucleotide analysis to achieve significant increases in both sensitivity and resolution
AU - Molin, Laura
AU - Cristoni, Simone
AU - Crotti, Sara
AU - Bernardi, Luigi Rossi
AU - Seraglia, Roberta
AU - Traldi, Pietro
PY - 2008/11
Y1 - 2008/11
N2 - Spraying of oligonucleotide-matrix solutions through a stainless steel (ss) sieve (38 μm, 450 mesh) leads to the formation, on the matrix-assisted laser desorption/ionization (MALDI) sample holder, of uniformly distributed microcrystals, well separated from each other. When the resulting sample holder surface is irradiated by laser, abundant molecular species form, with a clear increase in both intensity and resolution with respect to values obtained by 'Dried Droplet', 'Double Layer', and 'Sandwich' deposition methods. In addition, unlike the usual situation, the sample is perfectly homogeneous, and identical spectra are obtained by irradiating different areas. On one hand, the data indicate that this method is highly effective for oligonucleotide MALDI analysis, and on the other, that it can be validly employed for fully automated MALDI procedures.
AB - Spraying of oligonucleotide-matrix solutions through a stainless steel (ss) sieve (38 μm, 450 mesh) leads to the formation, on the matrix-assisted laser desorption/ionization (MALDI) sample holder, of uniformly distributed microcrystals, well separated from each other. When the resulting sample holder surface is irradiated by laser, abundant molecular species form, with a clear increase in both intensity and resolution with respect to values obtained by 'Dried Droplet', 'Double Layer', and 'Sandwich' deposition methods. In addition, unlike the usual situation, the sample is perfectly homogeneous, and identical spectra are obtained by irradiating different areas. On one hand, the data indicate that this method is highly effective for oligonucleotide MALDI analysis, and on the other, that it can be validly employed for fully automated MALDI procedures.
KW - MALDI sample preparation
KW - MALDI-MS
KW - Oligonucleotides
KW - Sample microspots
KW - Sieve-based device
UR - http://www.scopus.com/inward/record.url?scp=55249112034&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=55249112034&partnerID=8YFLogxK
U2 - 10.1002/jms.1428
DO - 10.1002/jms.1428
M3 - Article
C2 - 18636466
AN - SCOPUS:55249112034
VL - 43
SP - 1512
EP - 1520
JO - Biomedical Mass Spectrometry
JF - Biomedical Mass Spectrometry
SN - 1076-5174
IS - 11
ER -