Sieve-based device for MALDI sample preparation. II. Instrumental parameterization

Simone Cristoni, Laura Molin, Sara Crotti, Luigi Rossi Bernardi, Roberta Seraglia, Pietro Traldi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A simple device effective for matrix-assisted laser desorption/ionization (MALDI) sample preparation, based on the spraying of matrix/sample solution through a stainless steel sieve (sieve-based device, SBD), has been employed for the preparation of MALDI samples of peptides, polysaccharides and high molecular weight proteins. The instrumental parameters, mainly the sprayer voltage, solution and auxiliary gas flows, and distances between sprayer-sieve and sieve-MALDI sample holder have been optimized. The MALDI spectra obtained by laser irradiation of the microspots so obtained, exhibit resolution and sensitivity higher than those achieved by the commonly employed dried droplets (DD) method. Worth noting is the fact that, in contrast to what is observed in the DD method, identical spectra are obtained by irradiation of different areas of the MALDI sample, showing that by using the SBD the matrix/analyte ratio is constant over the entire surface.

Original languageEnglish
Pages (from-to)1579-1586
Number of pages8
JournalJournal of Mass Spectrometry
Volume44
Issue number11
DOIs
Publication statusPublished - Nov 2009

Fingerprint

sieves
Sieves
Parameterization
parameterization
Ionization
Desorption
Lasers
desorption
ionization
Equipment and Supplies
preparation
matrices
lasers
sprayers
Stainless Steel
irradiation
polysaccharides
spraying
Laser beam effects
Spraying

Keywords

  • MALDI
  • Proteins
  • Resolution increase
  • Saccharides
  • Sieve-based device

ASJC Scopus subject areas

  • Spectroscopy

Cite this

Sieve-based device for MALDI sample preparation. II. Instrumental parameterization. / Cristoni, Simone; Molin, Laura; Crotti, Sara; Bernardi, Luigi Rossi; Seraglia, Roberta; Traldi, Pietro.

In: Journal of Mass Spectrometry, Vol. 44, No. 11, 11.2009, p. 1579-1586.

Research output: Contribution to journalArticle

Cristoni, Simone ; Molin, Laura ; Crotti, Sara ; Bernardi, Luigi Rossi ; Seraglia, Roberta ; Traldi, Pietro. / Sieve-based device for MALDI sample preparation. II. Instrumental parameterization. In: Journal of Mass Spectrometry. 2009 ; Vol. 44, No. 11. pp. 1579-1586.
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