Signal requirements for activation of leukaemic T cells from a chronic lymphocytic leukaemia (T-CLL)

M. R. Zocchi, A. Poggi, S. Heltai, A. Villa, L. Inverardi, A. Vicari, M. G. Sabbadin, M. Ferrarini

Research output: Contribution to journalArticle

Abstract

In order to define the signal requirements for leukaemic T cell activation, the proliferation and interleukin-2 (IL-2) production of peripheral lymphocytes from a patient with a HTLV-I-, CD4+, CD45RA+ CD45RO+ CD25- T-CLL were evaluated after the delivery of different stimuli. Unlike resting CD4+ normal T lymphocytes that can be activated only by a two-signal stimulation, T-CLL cells proliferated and released IL-2 in response to a pair of anti-CD2 monoclonal antibodies (MoAbs) or concavanalin A (Con A) in the absence of both accessory cells (AC) and phorbol myristate acetate (PMA). The two stimuli were also able to induce CD25 expression within 12-20 h on the majority of T-CLL cells. A response to anti-CD3 and anti-CD28 MoAbs was detected only in the presence of PMA, similar to that observed in normal resting T lymphocytes matched for phenotype. Both Con A- and CD2-induced proliferation were strongly inhibited by the addition of anti-CD25 MoAb. Furthermore, T-CLL lymphocytes acquired anti-tumour lytic activity after culture in the presence of PMA and ionomycin. We conclude that HTLV1- CD25- T-CLL can be characterized not only by morphological and phenotypical studies but also on the basis of signal requirements for cell activation.

Original languageEnglish
Pages (from-to)108-113
Number of pages6
JournalClinical and Experimental Immunology
Volume82
Issue number1
Publication statusPublished - 1990

    Fingerprint

Keywords

  • activation
  • leukaemia
  • lymphocytes

ASJC Scopus subject areas

  • Immunology

Cite this