Signature of B-CLL with different prognosis by shrunken centroids of surface antigen expression profiling

Antonella Zucchetto, Paolo Sonego, Massimo Degan, Riccardo Bomben, Michele Dal Bo, Stefania Russo, Vincenza Attadia, Maurizio Rupolo, Francesco Buccisano, Maria Ilaria Del Principe, Giovanni Del Poeta, Carlo Pucillo, Alfonso Colombatti, Renato Campanini, Valter Gattei

Research output: Contribution to journalArticle

Abstract

With the aim of identifying the immunophenotypic profile of B-cell chronic lymphocytic leukemia (B-CLL) subsets with different prognosis, we investigated by flow cytometry the expression of 36 surface antigens in 123 cases, all with survivals. By analyzing results with unsupervised (hierarchical and K-means clustering) algorithms, three distinct immunophenotypic groups (I, II, and III) were identified, group I (51/123) with longer survivals, as compared to the group II (36/123) and III (36/123). The immunophenotypic signatures of these groups, as determined by applying the nearest Shrunken centroids method as class predictor, were characterized by the coordinated and differential expression of 12 surface markers, that is, group I: above-average expression of CD62L, CD54, CD49c, and CD25, below-average expression of CD38; group II: above-average expression of CD38, CD49d, CD29, and CD49e; and group III: below-average expression of the above markers, overexpression of CD23, CD20, SmIg, and CD79b. As opposed to groups II-III, group I B-CLLs lacked expression of ZAP-70 and activation-induced cytidinedeaminase in the majority of cases, while more frequently had mutated IgVH genes and IgVH mutations consistent with antigen-driven selection. Our findings contribute to improve the immunophenotypical identification of disease subsets with different prognosis and suggest a set of surface antigens to be employed as prognosticators in routine diagnostic/prognostic procedures.

Original languageEnglish
Pages (from-to)113-123
Number of pages11
JournalJournal of Cellular Physiology
Volume204
Issue number1
DOIs
Publication statusPublished - Jul 2005

    Fingerprint

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Zucchetto, A., Sonego, P., Degan, M., Bomben, R., Dal Bo, M., Russo, S., Attadia, V., Rupolo, M., Buccisano, F., Del Principe, M. I., Del Poeta, G., Pucillo, C., Colombatti, A., Campanini, R., & Gattei, V. (2005). Signature of B-CLL with different prognosis by shrunken centroids of surface antigen expression profiling. Journal of Cellular Physiology, 204(1), 113-123. https://doi.org/10.1002/jcp.20269