Abstract
IL-9, which may be an inflammatory or regulatory cytokine, can be experimentally produced in a Th17 or modified Th2 context in the presence of T cell receptor (TCR) stimulation. The primary aim of this study was to measure serum IL-9 levels in patients with inflammatory bowel disease (IBD), and evaluate their relationships with the patients' clinical characteristics. The secondary aim was to determine the levels of interferon-3 (IFN (interferon)-3), Th2 cytokines (IL-4, IL-5 and IL-13), and IL-6 in order to clarify the context of detectable peripheral cytokines in which IL-9 is produced. Venous blood samples of 43 IBD patients (20 with Crohn's disease [CD] and 23 with ulcerative colitis [UC]) were analysed by means of quantitative enzyme-linked immunosorbent assays using purified anti-human IL-4, IL-5, IL-13, IFN-3, IL-9 and IL-6 antibodies, and the laboratory findings were statistically correlated with their clinical expression. None of the patients showed the peripheral presence of IL-4, IL-5 and IL-13. Forty (93%) were positive for IFN-3, thus confirming the presence of Th1 in both UC and CD, and IFN-3 levels correlated with disease activity (P = 0.045). Eighteen patients (41%) were positive for IL-9, which was associated with a severe prognosis (P
| Original language | English |
|---|---|
| Pages (from-to) | 569-575 |
| Number of pages | 7 |
| Journal | International Journal of Immunopathology and Pharmacology |
| Volume | 28 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Dec 1 2015 |
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Keywords
- inflammation
- inflammatory bowel disease
- interleukin
- lymphocyte homing
- lymphocytes
- mucosal immunity
ASJC Scopus subject areas
- Pharmacology
- Immunology
- Immunology and Allergy
Cite this
Significance of serum Il-9 levels in inflammatory bowel disease. / Defendenti, Caterina; Sarzi-Puttini, Piercarlo; Saibeni, Simone; Bollani, Simona; Bruno, Savino; Almasio, Piero Luigi; Declich, Paolo; Atzeni, Fabiola.
In: International Journal of Immunopathology and Pharmacology, Vol. 28, No. 4, 01.12.2015, p. 569-575.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Significance of serum Il-9 levels in inflammatory bowel disease
AU - Defendenti, Caterina
AU - Sarzi-Puttini, Piercarlo
AU - Saibeni, Simone
AU - Bollani, Simona
AU - Bruno, Savino
AU - Almasio, Piero Luigi
AU - Declich, Paolo
AU - Atzeni, Fabiola
PY - 2015/12/1
Y1 - 2015/12/1
N2 - IL-9, which may be an inflammatory or regulatory cytokine, can be experimentally produced in a Th17 or modified Th2 context in the presence of T cell receptor (TCR) stimulation. The primary aim of this study was to measure serum IL-9 levels in patients with inflammatory bowel disease (IBD), and evaluate their relationships with the patients' clinical characteristics. The secondary aim was to determine the levels of interferon-3 (IFN (interferon)-3), Th2 cytokines (IL-4, IL-5 and IL-13), and IL-6 in order to clarify the context of detectable peripheral cytokines in which IL-9 is produced. Venous blood samples of 43 IBD patients (20 with Crohn's disease [CD] and 23 with ulcerative colitis [UC]) were analysed by means of quantitative enzyme-linked immunosorbent assays using purified anti-human IL-4, IL-5, IL-13, IFN-3, IL-9 and IL-6 antibodies, and the laboratory findings were statistically correlated with their clinical expression. None of the patients showed the peripheral presence of IL-4, IL-5 and IL-13. Forty (93%) were positive for IFN-3, thus confirming the presence of Th1 in both UC and CD, and IFN-3 levels correlated with disease activity (P = 0.045). Eighteen patients (41%) were positive for IL-9, which was associated with a severe prognosis (P
AB - IL-9, which may be an inflammatory or regulatory cytokine, can be experimentally produced in a Th17 or modified Th2 context in the presence of T cell receptor (TCR) stimulation. The primary aim of this study was to measure serum IL-9 levels in patients with inflammatory bowel disease (IBD), and evaluate their relationships with the patients' clinical characteristics. The secondary aim was to determine the levels of interferon-3 (IFN (interferon)-3), Th2 cytokines (IL-4, IL-5 and IL-13), and IL-6 in order to clarify the context of detectable peripheral cytokines in which IL-9 is produced. Venous blood samples of 43 IBD patients (20 with Crohn's disease [CD] and 23 with ulcerative colitis [UC]) were analysed by means of quantitative enzyme-linked immunosorbent assays using purified anti-human IL-4, IL-5, IL-13, IFN-3, IL-9 and IL-6 antibodies, and the laboratory findings were statistically correlated with their clinical expression. None of the patients showed the peripheral presence of IL-4, IL-5 and IL-13. Forty (93%) were positive for IFN-3, thus confirming the presence of Th1 in both UC and CD, and IFN-3 levels correlated with disease activity (P = 0.045). Eighteen patients (41%) were positive for IL-9, which was associated with a severe prognosis (P
KW - inflammation
KW - inflammatory bowel disease
KW - interleukin
KW - lymphocyte homing
KW - lymphocytes
KW - mucosal immunity
UR - http://www.scopus.com/inward/record.url?scp=84946090320&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84946090320&partnerID=8YFLogxK
U2 - 10.1177/0394632015600535
DO - 10.1177/0394632015600535
M3 - Article
C2 - 26377844
AN - SCOPUS:84946090320
VL - 28
SP - 569
EP - 575
JO - International Journal of Immunopathology and Pharmacology
JF - International Journal of Immunopathology and Pharmacology
SN - 0394-6320
IS - 4
ER -