Significance of serum Il-9 levels in inflammatory bowel disease

Caterina Defendenti, Piercarlo Sarzi-Puttini, Simone Saibeni, Simona Bollani, Savino Bruno, Piero Luigi Almasio, Paolo Declich, Fabiola Atzeni

Research output: Contribution to journalArticle

Abstract

IL-9, which may be an inflammatory or regulatory cytokine, can be experimentally produced in a Th17 or modified Th2 context in the presence of T cell receptor (TCR) stimulation. The primary aim of this study was to measure serum IL-9 levels in patients with inflammatory bowel disease (IBD), and evaluate their relationships with the patients' clinical characteristics. The secondary aim was to determine the levels of interferon-3 (IFN (interferon)-3), Th2 cytokines (IL-4, IL-5 and IL-13), and IL-6 in order to clarify the context of detectable peripheral cytokines in which IL-9 is produced. Venous blood samples of 43 IBD patients (20 with Crohn's disease [CD] and 23 with ulcerative colitis [UC]) were analysed by means of quantitative enzyme-linked immunosorbent assays using purified anti-human IL-4, IL-5, IL-13, IFN-3, IL-9 and IL-6 antibodies, and the laboratory findings were statistically correlated with their clinical expression. None of the patients showed the peripheral presence of IL-4, IL-5 and IL-13. Forty (93%) were positive for IFN-3, thus confirming the presence of Th1 in both UC and CD, and IFN-3 levels correlated with disease activity (P = 0.045). Eighteen patients (41%) were positive for IL-9, which was associated with a severe prognosis (P

Original languageEnglish
Pages (from-to)569-575
Number of pages7
JournalInternational Journal of Immunopathology and Pharmacology
Volume28
Issue number4
DOIs
Publication statusPublished - Dec 1 2015

Fingerprint

Interleukin-9
Inflammatory Bowel Diseases
Interleukin-13
Interleukin-5
Interleukin-4
Serum
Cytokines
Ulcerative Colitis
Crohn Disease
Interleukin-6
T-Cell Antigen Receptor
Interferons
Enzyme-Linked Immunosorbent Assay
Antibodies

Keywords

  • inflammation
  • inflammatory bowel disease
  • interleukin
  • lymphocyte homing
  • lymphocytes
  • mucosal immunity

ASJC Scopus subject areas

  • Pharmacology
  • Immunology
  • Immunology and Allergy

Cite this

Defendenti, C., Sarzi-Puttini, P., Saibeni, S., Bollani, S., Bruno, S., Almasio, P. L., ... Atzeni, F. (2015). Significance of serum Il-9 levels in inflammatory bowel disease. International Journal of Immunopathology and Pharmacology, 28(4), 569-575. https://doi.org/10.1177/0394632015600535

Significance of serum Il-9 levels in inflammatory bowel disease. / Defendenti, Caterina; Sarzi-Puttini, Piercarlo; Saibeni, Simone; Bollani, Simona; Bruno, Savino; Almasio, Piero Luigi; Declich, Paolo; Atzeni, Fabiola.

In: International Journal of Immunopathology and Pharmacology, Vol. 28, No. 4, 01.12.2015, p. 569-575.

Research output: Contribution to journalArticle

Defendenti, C, Sarzi-Puttini, P, Saibeni, S, Bollani, S, Bruno, S, Almasio, PL, Declich, P & Atzeni, F 2015, 'Significance of serum Il-9 levels in inflammatory bowel disease', International Journal of Immunopathology and Pharmacology, vol. 28, no. 4, pp. 569-575. https://doi.org/10.1177/0394632015600535
Defendenti, Caterina ; Sarzi-Puttini, Piercarlo ; Saibeni, Simone ; Bollani, Simona ; Bruno, Savino ; Almasio, Piero Luigi ; Declich, Paolo ; Atzeni, Fabiola. / Significance of serum Il-9 levels in inflammatory bowel disease. In: International Journal of Immunopathology and Pharmacology. 2015 ; Vol. 28, No. 4. pp. 569-575.
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