Purpose: To define the biological features of the variable aggressiveness of cholesteatoma, we performed a retrospective cytogenetic and kinetic study. Materials and Methods: We studied 44 cases of cholesteatoma by using fluorescence in situ hybridization with specific (α-satellite DNA probes for chromosome 7 and immunohistochemical analysis of proliferating cell nuclear antigen (PCNA) with PC10 monoclonal antibody. Results: We observed the presence of three or four chromosome 7 in elevated percentages of cells of 23 cases, and a high PCNA index (above mean value) in 22 cases. Conclusion: Based on our results, we maintain that the presence of an extra chromosome 7 is correlated with an increase of proliferative activity. Therefore, trisomy 7 can be considered a prognostic marker and an indicator of aggressive behavior in cholesteatoma.
|Number of pages||4|
|Journal||American Journal of Otolaryngology - Head and Neck Medicine and Surgery|
|Publication status||Published - Mar 1998|
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