TY - JOUR
T1 - Significance of trisomy 7 related to PCNA index in cholesteatoma
AU - Lavezzi, Anna
AU - Mantovani, Mario
AU - Cazzullo, Alessandra
AU - Turconi, Paola
AU - Matturri, Luigi
PY - 1998/3
Y1 - 1998/3
N2 - Purpose: To define the biological features of the variable aggressiveness of cholesteatoma, we performed a retrospective cytogenetic and kinetic study. Materials and Methods: We studied 44 cases of cholesteatoma by using fluorescence in situ hybridization with specific (α-satellite DNA probes for chromosome 7 and immunohistochemical analysis of proliferating cell nuclear antigen (PCNA) with PC10 monoclonal antibody. Results: We observed the presence of three or four chromosome 7 in elevated percentages of cells of 23 cases, and a high PCNA index (above mean value) in 22 cases. Conclusion: Based on our results, we maintain that the presence of an extra chromosome 7 is correlated with an increase of proliferative activity. Therefore, trisomy 7 can be considered a prognostic marker and an indicator of aggressive behavior in cholesteatoma.
AB - Purpose: To define the biological features of the variable aggressiveness of cholesteatoma, we performed a retrospective cytogenetic and kinetic study. Materials and Methods: We studied 44 cases of cholesteatoma by using fluorescence in situ hybridization with specific (α-satellite DNA probes for chromosome 7 and immunohistochemical analysis of proliferating cell nuclear antigen (PCNA) with PC10 monoclonal antibody. Results: We observed the presence of three or four chromosome 7 in elevated percentages of cells of 23 cases, and a high PCNA index (above mean value) in 22 cases. Conclusion: Based on our results, we maintain that the presence of an extra chromosome 7 is correlated with an increase of proliferative activity. Therefore, trisomy 7 can be considered a prognostic marker and an indicator of aggressive behavior in cholesteatoma.
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U2 - 10.1016/S0196-0709(98)90105-3
DO - 10.1016/S0196-0709(98)90105-3
M3 - Article
C2 - 9550442
AN - SCOPUS:0031940621
VL - 19
SP - 109
EP - 112
JO - American Journal of Otolaryngology - Head and Neck Medicine and Surgery
JF - American Journal of Otolaryngology - Head and Neck Medicine and Surgery
SN - 0196-0709
IS - 2
ER -