Abstract
RIZ1 isoform, but not RIZ2, is commonly silenced in many types of tumors. In osteosarcoma cells, RIZ1 protein is very abundant. The silencing of YY1 protein, a recent target gene in osteosarcoma cells, reduced the expression of RIZ1 protein. Here we show that RIZ1 overexpression is a transcriptional event documented by Western blot, RT-PCR, and promoter assays. YY1 protein binds and cooperates to positive regulation of the RIZ1 promoter and its presence reduced the dimethyl lysine 9 histone 3 by chromatin immunoprecipitation assays. These results indicate that overexpression of YY1 in osteosarcoma cells plays a key role in positive regulation of RIZ1. The coexpression of RIZ1/YY1 proteins suggests a tandem regulatory mechanism in human osteosarcoma cells and tissues.
Original language | English |
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Pages (from-to) | 33-41 |
Number of pages | 9 |
Journal | Oncology Research |
Volume | 17 |
Issue number | 1 |
Publication status | Published - 2008 |
Keywords
- Cancer
- Ostosarcoma
- RIZ
- YY1
ASJC Scopus subject areas
- Cancer Research