Silencing of YY1 downregulates RIZ1 promoter in human osteosarcoma

Ciro Abbondanza, Filomena De Nigris, Caterina De Rosa, Raffaele Rossiello, Giovanni Alfredo Puca, Claudio Napoli

Research output: Contribution to journalArticlepeer-review

Abstract

RIZ1 isoform, but not RIZ2, is commonly silenced in many types of tumors. In osteosarcoma cells, RIZ1 protein is very abundant. The silencing of YY1 protein, a recent target gene in osteosarcoma cells, reduced the expression of RIZ1 protein. Here we show that RIZ1 overexpression is a transcriptional event documented by Western blot, RT-PCR, and promoter assays. YY1 protein binds and cooperates to positive regulation of the RIZ1 promoter and its presence reduced the dimethyl lysine 9 histone 3 by chromatin immunoprecipitation assays. These results indicate that overexpression of YY1 in osteosarcoma cells plays a key role in positive regulation of RIZ1. The coexpression of RIZ1/YY1 proteins suggests a tandem regulatory mechanism in human osteosarcoma cells and tissues.

Original languageEnglish
Pages (from-to)33-41
Number of pages9
JournalOncology Research
Volume17
Issue number1
Publication statusPublished - 2008

Keywords

  • Cancer
  • Ostosarcoma
  • RIZ
  • YY1

ASJC Scopus subject areas

  • Cancer Research

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