Silver nanoparticles inhaled during pregnancy reach and affect the placenta and the foetus

Luisa Campagnolo, Micol Massimiani, Lucia Vecchione, Diletta Piccirilli, Nicola Toschi, Andrea Magrini, Elena Bonanno, Manuel Scimeca, Luca Castagnozzi, Giorgio Buonanno, Luca Stabile, Francesco Cubadda, Federica Aureli, Paul H.B. Fokkens, Wolfgang G. Kreyling, Flemming R. Cassee, Antonio Pietroiusti

Research output: Contribution to journalArticle

Abstract

Recently, interest for the potential impact of consumer-relevant engineered nanoparticles on pregnancy has dramatically increased. This study investigates whether inhaled silver nanoparticles (AgNPs) reach and cross mouse placental barrier and induce adverse effects. Apart from their relevance for the growing use in consumer products and biomedical applications, AgNPs are selected since they can be unequivocally identified in tissues. Pregnant mouse females are exposed during the first 15 days of gestation by nose-only inhalation to a freshly produced aerosol of 18–20 nm AgNPs for either 1 or 4 h, at a particle number concentration of 3.80 × 107 part./cm−3 and at a mass concentration of 640 μg/m³. AgNPs are identified and quantitated in maternal tissues, placentas and foetuses by transmission electron microscopy coupled with energy-dispersive X-ray spectroscopy and single-particle inductively coupled plasma mass spectrometry. Inhalation of AgNPs results in increased number of resorbed foetuses associated with reduced oestrogen plasma levels, in the 4 h/day exposed mothers. Increased expression of pregnancy-relevant inflammatory cytokines is also detected in the placentas of both groups. These results prove that NPs are able to reach and cross the mouse placenta and suggest that precaution should be taken with respect to acute exposure to nanoparticles during pregnancy.

Original languageEnglish
Pages (from-to)687-698
Number of pages12
JournalNanotoxicology
Volume11
Issue number5
DOIs
Publication statusPublished - May 28 2017

Fingerprint

Silver
Nanoparticles
Placenta
Fetus
Pregnancy
Tissue
Inhalation
Inductively coupled plasma mass spectrometry
Consumer products
X-Ray Emission Spectrometry
Aerosols
Estrogens
Transmission Electron Microscopy
Nose
Cytokines
Transmission electron microscopy
Plasmas
Mass Spectrometry
Mothers

Keywords

  • embryo
  • inhalation exposure
  • placental barrier
  • pregnancy
  • Silver nanoparticles

ASJC Scopus subject areas

  • Biomedical Engineering
  • Toxicology

Cite this

Campagnolo, L., Massimiani, M., Vecchione, L., Piccirilli, D., Toschi, N., Magrini, A., ... Pietroiusti, A. (2017). Silver nanoparticles inhaled during pregnancy reach and affect the placenta and the foetus. Nanotoxicology, 11(5), 687-698. https://doi.org/10.1080/17435390.2017.1343875

Silver nanoparticles inhaled during pregnancy reach and affect the placenta and the foetus. / Campagnolo, Luisa; Massimiani, Micol; Vecchione, Lucia; Piccirilli, Diletta; Toschi, Nicola; Magrini, Andrea; Bonanno, Elena; Scimeca, Manuel; Castagnozzi, Luca; Buonanno, Giorgio; Stabile, Luca; Cubadda, Francesco; Aureli, Federica; Fokkens, Paul H.B.; Kreyling, Wolfgang G.; Cassee, Flemming R.; Pietroiusti, Antonio.

In: Nanotoxicology, Vol. 11, No. 5, 28.05.2017, p. 687-698.

Research output: Contribution to journalArticle

Campagnolo, L, Massimiani, M, Vecchione, L, Piccirilli, D, Toschi, N, Magrini, A, Bonanno, E, Scimeca, M, Castagnozzi, L, Buonanno, G, Stabile, L, Cubadda, F, Aureli, F, Fokkens, PHB, Kreyling, WG, Cassee, FR & Pietroiusti, A 2017, 'Silver nanoparticles inhaled during pregnancy reach and affect the placenta and the foetus', Nanotoxicology, vol. 11, no. 5, pp. 687-698. https://doi.org/10.1080/17435390.2017.1343875
Campagnolo L, Massimiani M, Vecchione L, Piccirilli D, Toschi N, Magrini A et al. Silver nanoparticles inhaled during pregnancy reach and affect the placenta and the foetus. Nanotoxicology. 2017 May 28;11(5):687-698. https://doi.org/10.1080/17435390.2017.1343875
Campagnolo, Luisa ; Massimiani, Micol ; Vecchione, Lucia ; Piccirilli, Diletta ; Toschi, Nicola ; Magrini, Andrea ; Bonanno, Elena ; Scimeca, Manuel ; Castagnozzi, Luca ; Buonanno, Giorgio ; Stabile, Luca ; Cubadda, Francesco ; Aureli, Federica ; Fokkens, Paul H.B. ; Kreyling, Wolfgang G. ; Cassee, Flemming R. ; Pietroiusti, Antonio. / Silver nanoparticles inhaled during pregnancy reach and affect the placenta and the foetus. In: Nanotoxicology. 2017 ; Vol. 11, No. 5. pp. 687-698.
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