Simian virus 40 sequences in human lymphoblastoid B-cell lines

Riccardo Dolcetti, Fernanda Martini, Michele Quaia, Annunziata Gloghini, Beatrice Vignocchi, Roberta Cariati, Marcella Martinelli, Antonino Carbone, Mauro Boiocchi, Mauro Tognon

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Abstract

Human Epstein-Barr virus-immortalized lymphoblastoid B-cell lines tested positive by PCR for simian virus 40 (SV40) DNA (22 of 42 cell lines, 52.3%). B lymphocytes or tissues from which B-cell lines derived were also SV40 positive. In situ hybridization showed that SV40 DNA was present in the nucleus of a small fraction (1/250) of cells. SV40 T-antigen mRNA was detected by reverse transcription-PCR. Lymphoblastoid B-cell lines (n = 4) infected with SV40 remained SV40 positive for 4 to 6 months. SV40-positive B-cell lines were more tumorigenic in SCID mice than were SV40-negative cell lines (4 of 5 [80%] SV40-positive cell lines versus 2 of 4 [50%] SV40-negative cell lines). These results suggest that SV40 may play a role in the early phases of human lymphomagenesis.

Original languageEnglish
Pages (from-to)1595-1597
Number of pages3
JournalJournal of Virology
Volume77
Issue number2
DOIs
Publication statusPublished - Jan 2003

ASJC Scopus subject areas

  • Immunology

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    Dolcetti, R., Martini, F., Quaia, M., Gloghini, A., Vignocchi, B., Cariati, R., Martinelli, M., Carbone, A., Boiocchi, M., & Tognon, M. (2003). Simian virus 40 sequences in human lymphoblastoid B-cell lines. Journal of Virology, 77(2), 1595-1597. https://doi.org/10.1128/JVI.77.2.1595-1597.2003