Simple and effective determination of apolipoprotein E genotypes by positive/negative polymerase chain reaction products

Davide Seripa, Emanuela Signori, Carolina Gravina, Maria Giovanna Matera, Monica Rinaldi, Vito M. Fazio

Research output: Contribution to journalArticle

Abstract

Several protein and DNA-based methods have been previously described for the identification of apolipoprotein E isoforms or genotypes. However, all of them generate frequently false-positive results. The purpose of this study was to set up a new, simple, and effective method for the analysis of the apoE polymorphism. A total of 1253 subjects previously examined for the apolipoprotein E polymorphism by restriction fragment length polymorphism were reanalyzed by our new method based on Taq DNA polymerase's inability to correctly initiate the replication in the presence of a mismatch at the 3′ end of the primer. We conceived a combination of 4 specific primers in 3 different pairs sharing the same stringent polymerase chain reaction conditions to directly detect the presence/absence of polymerase chain reaction products, and thus reveal the 6 apolipoprotein E genotypes. We confirm our previous results in 1171 subjects, whereas in 82 subjects out of 1253 (about 6%), the results have been reinterpreted. The final analysis revealed a total of 12 homozygotic subjects for the e2 allele (1.0%), 874 homozygotes for the e3 allele (69.8 %), and 8 homozygotes for the e4 allele (0.6 %). The frequence of heterozygotes was 8.7% for the e2/e3 genotype (n=109), 1.4% for the e2/e4 genotype (n=17), and 0.6% for the e3/e4 genotype (n=8). Relative allele frequencies were e2=0.060, e3=0.834, and e4=0.106. We describe a new, simple, unequivocal, and nonexpensive method for the identification of the 6 apoE genotypes.

Original languageEnglish
Pages (from-to)180-185
Number of pages6
JournalDiagnostic Molecular Pathology
Volume15
Issue number3
DOIs
Publication statusPublished - Sep 2006

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Keywords

  • Allele-specific PCR
  • Apolipoprotein E
  • Genotyping
  • Polymorphism
  • Primer mismatch

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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