Simple and rapid liquid chromatographic-turbo ion spray mass spectrometric determination of topiramate in human plasma

Manuela Contin, Roberto Riva, Fiorenzo Albani, Agostino Baruzzi

Research output: Contribution to journalArticlepeer-review

Abstract

We present a simple and fast method for the determination of the novel antiepileptic drug topiramate in human plasma by high-performance liquid chromatography coupled with turbo ion spray mass spectrometry. Plasma sample pre-treatment was based on simple deproteinization by acetonitrile. Liquid chromatographic analysis was carried out on a reversed-phase column (C18, 125×4 mm I.D., 5 μm) using acetonitrile-ammonium acetate buffer, pH 6.3 as the mobile phase, at a flow-rate of 0.8 ml/min. Retention time for topiramate was 2.1 min. The detector was a single quadrupole mass spectrometer coupled to a turbo ion spray ion source and a heated nebulizer probe, operating in the positive ion mode. Ion source temperature was off; voltage was +5800 V; nebulizer and curtain gas flow-rates were 6 and 10 ml/min, respectively. Calibration curves for topiramate were linear over the range 1 to 20 μg/ml. Absolute recovery ranged between 92 and 95%. Intra- and inter-assay precision was

Original languageEnglish
Pages (from-to)133-137
Number of pages5
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume761
Issue number1
DOIs
Publication statusPublished - Sep 15 2001

Keywords

  • Topiramate

ASJC Scopus subject areas

  • Chemistry(all)

Fingerprint Dive into the research topics of 'Simple and rapid liquid chromatographic-turbo ion spray mass spectrometric determination of topiramate in human plasma'. Together they form a unique fingerprint.

Cite this