Abstract
Two amplification steps were made to detect Pneumocystis carinii DNA by polymerase chain reaction (PCR). pAZ102-E and pAZ102-H (standard PCR), pAZ102-L2 (sense), and pAZ102-E (antisense) (two-step PCR) were used as primers. The amplification products were analyzed by ethidium bromide. After the two-step PCR, ethidium bromide detected all samples positive by oligohybridization after one amplification step. Our two-step PCR is a rapid, cost-effective, and clinically suitable method for the detection of P. carinii infection.
Original language | English |
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Pages (from-to) | 2788-2789 |
Number of pages | 2 |
Journal | Journal of Clinical Microbiology |
Volume | 31 |
Issue number | 10 |
Publication status | Published - 1993 |
ASJC Scopus subject areas
- Microbiology
- Microbiology (medical)