Simple and rapid two-step polymerase chain reaction for diagnosis of Pneumocystis carinii infection

E. Tamburrini, P. Mencarini, A. De Luca, A. Antinori, E. Visconti, A. Ammassari, L. Ortona, E. Ortona, A. Siracusano, G. Vicari

Research output: Contribution to journalArticlepeer-review

Abstract

Two amplification steps were made to detect Pneumocystis carinii DNA by polymerase chain reaction (PCR). pAZ102-E and pAZ102-H (standard PCR), pAZ102-L2 (sense), and pAZ102-E (antisense) (two-step PCR) were used as primers. The amplification products were analyzed by ethidium bromide. After the two-step PCR, ethidium bromide detected all samples positive by oligohybridization after one amplification step. Our two-step PCR is a rapid, cost-effective, and clinically suitable method for the detection of P. carinii infection.

Original languageEnglish
Pages (from-to)2788-2789
Number of pages2
JournalJournal of Clinical Microbiology
Volume31
Issue number10
Publication statusPublished - 1993

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

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