Simple and rapid two-step polymerase chain reaction for diagnosis of Pneumocystis carinii infection

E. Tamburrini; P. Mencarini; A. De Luca; A. Antinori; E. Visconti; A. Ammassari; L. Ortona; E. Ortona; A. Siracusano; G. Vicari

Simple and rapid two-step polymerase chain reaction for diagnosis of Pneumocystis carinii infection

E. Tamburrini, P. Mencarini, A. De Luca, A. Antinori, E. Visconti, A. Ammassari, L. Ortona, E. Ortona, A. Siracusano, G. Vicari

Istituto Nazionale per le Malattie Infettive Lazzaro Spallanzani

Research output: Contribution to journal › Article › peer-review

Abstract

Two amplification steps were made to detect Pneumocystis carinii DNA by polymerase chain reaction (PCR). pAZ102-E and pAZ102-H (standard PCR), pAZ102-L2 (sense), and pAZ102-E (antisense) (two-step PCR) were used as primers. The amplification products were analyzed by ethidium bromide. After the two-step PCR, ethidium bromide detected all samples positive by oligohybridization after one amplification step. Our two-step PCR is a rapid, cost-effective, and clinically suitable method for the detection of P. carinii infection.

Original language	English
Pages (from-to)	2788-2789
Number of pages	2
Journal	Journal of Clinical Microbiology
Volume	31
Issue number	10
Publication status	Published - 1993

ASJC Scopus subject areas

Microbiology
Microbiology (medical)

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Simple and rapid two-step polymerase chain reaction for diagnosis of Pneumocystis carinii infection. / Tamburrini, E.; Mencarini, P.; De Luca, A.; Antinori, A.; Visconti, E.; Ammassari, A.; Ortona, L.; Ortona, E.; Siracusano, A.; Vicari, G.

In: Journal of Clinical Microbiology, Vol. 31, No. 10, 1993, p. 2788-2789.

Research output: Contribution to journal › Article › peer-review

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abstract = "Two amplification steps were made to detect Pneumocystis carinii DNA by polymerase chain reaction (PCR). pAZ102-E and pAZ102-H (standard PCR), pAZ102-L2 (sense), and pAZ102-E (antisense) (two-step PCR) were used as primers. The amplification products were analyzed by ethidium bromide. After the two-step PCR, ethidium bromide detected all samples positive by oligohybridization after one amplification step. Our two-step PCR is a rapid, cost-effective, and clinically suitable method for the detection of P. carinii infection.",

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AU - Mencarini, P.

AU - De Luca, A.

AU - Antinori, A.

AU - Visconti, E.

AU - Ammassari, A.

AU - Ortona, L.

AU - Ortona, E.

AU - Siracusano, A.

AU - Vicari, G.

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Simple and rapid two-step polymerase chain reaction for diagnosis of Pneumocystis carinii infection

Abstract

ASJC Scopus subject areas

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