Simple and rapid validated HPLC-fluorescence determination of perampanel in the plasma of patients with epilepsy

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3 Citations (Scopus)

Abstract

We present a simple and fast high-performance liquid chromatography method with fluorescence detection for the determination of the antiepileptic drug perampanel in human plasma. The chromatographic separation was performed on a Kinetex PFP (100 × 2.6 mm, 4.6 µm) column, using a mobile phase of sodium acetate 0.03 M pH 3.7 and acetonitrile (40/60, v/v), at a flow rate of 0.8 mL/min. Total chromatography time for each run was 5 min. Sample preparation (250 µL) involved only one simple precipitation step by acetonitrile spiked with mirtazapine as internal standard. The method was validated over a concentration range of 20–1000 ng/mL and successfully applied to measure perampanel concentrations in plasma samples obtained from patients with epilepsy. This assay combines the high specificity of fluorescence detection with a very simple and fast sample pretreatment and can offer real advantages over existing methods in terms of simplicity and transferability to a therapeutic drug monitoring setting.

Original languageEnglish
Pages (from-to)15-20
Number of pages6
JournalPractical Laboratory Medicine
Volume10
DOIs
Publication statusPublished - Mar 1 2018

Fingerprint

Epilepsy
Fluorescence
High Pressure Liquid Chromatography
Plasma (human)
Plasmas
Sodium Acetate
High performance liquid chromatography
Chromatography
Anticonvulsants
Assays
Drug Monitoring
Flow rate
Monitoring
Pharmaceutical Preparations
acetonitrile
perampanel
mirtazapine

Keywords

  • Antiepileptic drugs
  • Clinical pharmacokinetics
  • Epilepsy
  • HPLC-F
  • Perampanel

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology
  • Clinical Biochemistry

Cite this

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title = "Simple and rapid validated HPLC-fluorescence determination of perampanel in the plasma of patients with epilepsy",
abstract = "We present a simple and fast high-performance liquid chromatography method with fluorescence detection for the determination of the antiepileptic drug perampanel in human plasma. The chromatographic separation was performed on a Kinetex PFP (100 × 2.6 mm, 4.6 µm) column, using a mobile phase of sodium acetate 0.03 M pH 3.7 and acetonitrile (40/60, v/v), at a flow rate of 0.8 mL/min. Total chromatography time for each run was 5 min. Sample preparation (250 µL) involved only one simple precipitation step by acetonitrile spiked with mirtazapine as internal standard. The method was validated over a concentration range of 20–1000 ng/mL and successfully applied to measure perampanel concentrations in plasma samples obtained from patients with epilepsy. This assay combines the high specificity of fluorescence detection with a very simple and fast sample pretreatment and can offer real advantages over existing methods in terms of simplicity and transferability to a therapeutic drug monitoring setting.",
keywords = "Antiepileptic drugs, Clinical pharmacokinetics, Epilepsy, HPLC-F, Perampanel",
author = "Susan Mohamed and Carmina Candela and Roberto Riva and Manuela Contin",
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TY - JOUR

T1 - Simple and rapid validated HPLC-fluorescence determination of perampanel in the plasma of patients with epilepsy

AU - Mohamed, Susan

AU - Candela, Carmina

AU - Riva, Roberto

AU - Contin, Manuela

PY - 2018/3/1

Y1 - 2018/3/1

N2 - We present a simple and fast high-performance liquid chromatography method with fluorescence detection for the determination of the antiepileptic drug perampanel in human plasma. The chromatographic separation was performed on a Kinetex PFP (100 × 2.6 mm, 4.6 µm) column, using a mobile phase of sodium acetate 0.03 M pH 3.7 and acetonitrile (40/60, v/v), at a flow rate of 0.8 mL/min. Total chromatography time for each run was 5 min. Sample preparation (250 µL) involved only one simple precipitation step by acetonitrile spiked with mirtazapine as internal standard. The method was validated over a concentration range of 20–1000 ng/mL and successfully applied to measure perampanel concentrations in plasma samples obtained from patients with epilepsy. This assay combines the high specificity of fluorescence detection with a very simple and fast sample pretreatment and can offer real advantages over existing methods in terms of simplicity and transferability to a therapeutic drug monitoring setting.

AB - We present a simple and fast high-performance liquid chromatography method with fluorescence detection for the determination of the antiepileptic drug perampanel in human plasma. The chromatographic separation was performed on a Kinetex PFP (100 × 2.6 mm, 4.6 µm) column, using a mobile phase of sodium acetate 0.03 M pH 3.7 and acetonitrile (40/60, v/v), at a flow rate of 0.8 mL/min. Total chromatography time for each run was 5 min. Sample preparation (250 µL) involved only one simple precipitation step by acetonitrile spiked with mirtazapine as internal standard. The method was validated over a concentration range of 20–1000 ng/mL and successfully applied to measure perampanel concentrations in plasma samples obtained from patients with epilepsy. This assay combines the high specificity of fluorescence detection with a very simple and fast sample pretreatment and can offer real advantages over existing methods in terms of simplicity and transferability to a therapeutic drug monitoring setting.

KW - Antiepileptic drugs

KW - Clinical pharmacokinetics

KW - Epilepsy

KW - HPLC-F

KW - Perampanel

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JO - Practical Laboratory Medicine

JF - Practical Laboratory Medicine

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