Simple and rapid validated HPLC-fluorescence determination of perampanel in the plasma of patients with epilepsy

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Abstract

We present a simple and fast high-performance liquid chromatography method with fluorescence detection for the determination of the antiepileptic drug perampanel in human plasma. The chromatographic separation was performed on a Kinetex PFP (100 × 2.6 mm, 4.6 µm) column, using a mobile phase of sodium acetate 0.03 M pH 3.7 and acetonitrile (40/60, v/v), at a flow rate of 0.8 mL/min. Total chromatography time for each run was 5 min. Sample preparation (250 µL) involved only one simple precipitation step by acetonitrile spiked with mirtazapine as internal standard. The method was validated over a concentration range of 20–1000 ng/mL and successfully applied to measure perampanel concentrations in plasma samples obtained from patients with epilepsy. This assay combines the high specificity of fluorescence detection with a very simple and fast sample pretreatment and can offer real advantages over existing methods in terms of simplicity and transferability to a therapeutic drug monitoring setting.

Original languageEnglish
Pages (from-to)15-20
Number of pages6
JournalPractical Laboratory Medicine
Volume10
DOIs
Publication statusPublished - Mar 1 2018

Keywords

  • Antiepileptic drugs
  • Clinical pharmacokinetics
  • Epilepsy
  • HPLC-F
  • Perampanel

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology
  • Clinical Biochemistry

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