Abstract
Analysis of families with germline p53 mutations shows that the mutant p53 allele behaves as a dominant oncogene at the genetic level, although it behaves as a recessive oncogene at the cellular level, since tumours invariably show mutation or loss of both wild-type alleles. At the biochemical level it is possible that some clinically important mutant p53 proteins may be carcinogenic through a dominant mechanism. We show that p53 mutants can be readily classified according to their dominant potential using a simple yeast functional assay. Wild-type p53 is constitutively expressed from a TRP1 vector, p53 mutants are expressed from an otherwise identical LEU2 vector and net transcriptional activity is scored using an ADE2-based reporter. Twenty seven p53 mutants were tested: 19 were recessive, i.e. gave white colonies, and eight showed dominant activity, i.e. gave pink/red colonies. This simple assay should facilitate studies on p53 dominance.
| Original language | English |
|---|---|
| Pages (from-to) | 2019-2021 |
| Number of pages | 3 |
| Journal | Carcinogenesis |
| Volume | 18 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - Oct 1997 |
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ASJC Scopus subject areas
- Cancer Research
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Simple identification of dominant p53 mutants by a yeast functional assay. / Inga, Alberto; Cresta, Sara; Monti, Paola; Aprile, Anna; Scott, Gina; Abbondandolo, Angelo; Iggo, Richard; Fronza, Gilberto.
In: Carcinogenesis, Vol. 18, No. 10, 10.1997, p. 2019-2021.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Simple identification of dominant p53 mutants by a yeast functional assay
AU - Inga, Alberto
AU - Cresta, Sara
AU - Monti, Paola
AU - Aprile, Anna
AU - Scott, Gina
AU - Abbondandolo, Angelo
AU - Iggo, Richard
AU - Fronza, Gilberto
PY - 1997/10
Y1 - 1997/10
N2 - Analysis of families with germline p53 mutations shows that the mutant p53 allele behaves as a dominant oncogene at the genetic level, although it behaves as a recessive oncogene at the cellular level, since tumours invariably show mutation or loss of both wild-type alleles. At the biochemical level it is possible that some clinically important mutant p53 proteins may be carcinogenic through a dominant mechanism. We show that p53 mutants can be readily classified according to their dominant potential using a simple yeast functional assay. Wild-type p53 is constitutively expressed from a TRP1 vector, p53 mutants are expressed from an otherwise identical LEU2 vector and net transcriptional activity is scored using an ADE2-based reporter. Twenty seven p53 mutants were tested: 19 were recessive, i.e. gave white colonies, and eight showed dominant activity, i.e. gave pink/red colonies. This simple assay should facilitate studies on p53 dominance.
AB - Analysis of families with germline p53 mutations shows that the mutant p53 allele behaves as a dominant oncogene at the genetic level, although it behaves as a recessive oncogene at the cellular level, since tumours invariably show mutation or loss of both wild-type alleles. At the biochemical level it is possible that some clinically important mutant p53 proteins may be carcinogenic through a dominant mechanism. We show that p53 mutants can be readily classified according to their dominant potential using a simple yeast functional assay. Wild-type p53 is constitutively expressed from a TRP1 vector, p53 mutants are expressed from an otherwise identical LEU2 vector and net transcriptional activity is scored using an ADE2-based reporter. Twenty seven p53 mutants were tested: 19 were recessive, i.e. gave white colonies, and eight showed dominant activity, i.e. gave pink/red colonies. This simple assay should facilitate studies on p53 dominance.
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U2 - 10.1093/carcin/18.10.2019
DO - 10.1093/carcin/18.10.2019
M3 - Article
VL - 18
SP - 2019
EP - 2021
JO - Carcinogenesis
JF - Carcinogenesis
SN - 0143-3334
IS - 10
ER -