Simple identification of dominant p53 mutants by a yeast functional assay

Alberto Inga, Sara Cresta, Paola Monti, Anna Aprile, Gina Scott, Angelo Abbondandolo, Richard Iggo, Gilberto Fronza

Research output: Contribution to journalArticle

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Abstract

Analysis of families with germline p53 mutations shows that the mutant p53 allele behaves as a dominant oncogene at the genetic level, although it behaves as a recessive oncogene at the cellular level, since tumours invariably show mutation or loss of both wild-type alleles. At the biochemical level it is possible that some clinically important mutant p53 proteins may be carcinogenic through a dominant mechanism. We show that p53 mutants can be readily classified according to their dominant potential using a simple yeast functional assay. Wild-type p53 is constitutively expressed from a TRP1 vector, p53 mutants are expressed from an otherwise identical LEU2 vector and net transcriptional activity is scored using an ADE2-based reporter. Twenty seven p53 mutants were tested: 19 were recessive, i.e. gave white colonies, and eight showed dominant activity, i.e. gave pink/red colonies. This simple assay should facilitate studies on p53 dominance.

Original languageEnglish
Pages (from-to)2019-2021
Number of pages3
JournalCarcinogenesis
Volume18
Issue number10
DOIs
Publication statusPublished - Oct 1997

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Yeasts
Alleles
Germ-Line Mutation
Mutant Proteins
Tumor Suppressor Genes
Oncogenes
Mutation
Neoplasms

ASJC Scopus subject areas

  • Cancer Research

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Simple identification of dominant p53 mutants by a yeast functional assay. / Inga, Alberto; Cresta, Sara; Monti, Paola; Aprile, Anna; Scott, Gina; Abbondandolo, Angelo; Iggo, Richard; Fronza, Gilberto.

In: Carcinogenesis, Vol. 18, No. 10, 10.1997, p. 2019-2021.

Research output: Contribution to journalArticle

Inga, A, Cresta, S, Monti, P, Aprile, A, Scott, G, Abbondandolo, A, Iggo, R & Fronza, G 1997, 'Simple identification of dominant p53 mutants by a yeast functional assay', Carcinogenesis, vol. 18, no. 10, pp. 2019-2021. https://doi.org/10.1093/carcin/18.10.2019
Inga, Alberto ; Cresta, Sara ; Monti, Paola ; Aprile, Anna ; Scott, Gina ; Abbondandolo, Angelo ; Iggo, Richard ; Fronza, Gilberto. / Simple identification of dominant p53 mutants by a yeast functional assay. In: Carcinogenesis. 1997 ; Vol. 18, No. 10. pp. 2019-2021.
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