Simulated digestion for testing the stability of edible vaccine based on Cucumber Mosaic Virus (CMV) chimeric particle display Hepatitis C virus (HCV) peptide

Antonella Vitti, Maria Nuzzaci, Valentina Condelli, Pasquale Piazzolla

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Edible vaccines must survive digestive process and preserve the specific structure of the antigenic peptide to elicit effective immune response. The stability of a protein to digestive process can be predicted by subjecting it to the in vitro assay with simulated gastric fluid (SGF) and simulated intestinal fluid (SIF). Here, we describe the protocol of producing and using chimeric Cucumber mosaic virus (CMV) displaying Hepatitis C virus (HCV) derived peptide (R9) in double copy as an oral vaccine. Its stability after treatment with SGF and SIF and the preservation of the antigenic properties were verified by SDS-PAGE and immuno western blot techniques.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages41-56
Number of pages16
Volume1108
DOIs
Publication statusPublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1108
ISSN (Print)10643745

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ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Vitti, A., Nuzzaci, M., Condelli, V., & Piazzolla, P. (2014). Simulated digestion for testing the stability of edible vaccine based on Cucumber Mosaic Virus (CMV) chimeric particle display Hepatitis C virus (HCV) peptide. In Methods in Molecular Biology (Vol. 1108, pp. 41-56). (Methods in Molecular Biology; Vol. 1108). https://doi.org/10.1007/978-1-62703-751-8-3