Simulation of continuous blood O 2 equilibrium curve over physiological pH, DPG, and PCO 2 range

R. M. Winslow, M. Samaja, N. J. Winslow, L. Rossi-Bernardi, R. I. Shrager

Research output: Contribution to journalArticle

Abstract

We analyzed 56 O 2 equilibrium curves of fresh human blood, each from 0 to 150 Torr PO 2. The data were collected over ranges of values for the 2,3-diphosphoglyceric acid-to-hemoglobin concentrations ratio [DPG]/[Hb] of 0.2-2.7, for pH of 7.0-7.8, and for PCO 2 of 7-70 Torr. Each curve was characterized according to the Adair scheme for the stepwise oxygenation of Hb, and the resulting constants (α 1, α 2, α 3, α 4) were analyzed to allow the simulation of the entire O 2 equilibrium curve under any conditions of [DPG]/[Hb], pH, and PCO 2 in the specific range. This analysis provides a powerful tool to study the affinity of Hb for O 2 within the red blood cell and to predict the shape of the O 2 equilibrium curve in various physiological and pathological states. Other attempts to predict blood O 2 affinity have considered only P 50 (the PO 2 at one-half saturation with O 2) or have provided too little data for continuous simulations.

Original languageEnglish
Pages (from-to)524-529
Number of pages6
JournalJournal of Applied Physiology Respiratory Environmental and Exercise Physiology
Volume54
Issue number2
Publication statusPublished - 1983

ASJC Scopus subject areas

  • Physiology
  • Endocrinology

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