Simulation of functional tricuspid regurgitation using an isolated porcine heart model

Francesco Maisano, Maurizio Taramasso, Andrea Guidotti, Alberto Redaelli, Gianfranco B. Fiore, Guido Baroni, Paolo Patete, Ottavio Alfieri

Research output: Contribution to journalArticle

Abstract

Background and aim of the study: The results of tricuspid annuloplasty to treat functional tricuspid regurgitation (FTR) are sometimes suboptimal, and alternative techniques are needed. In the absence of reliable FTR models, and in an effort to minimize the need for animal experiments, a reproducible bench-model was developed of FTR, that allowed the simulation of the anatomic features of the condition. Methods: A fresh porcine heart was mounted on a rigid support that was placed into a basin filled with saline; a closed circuit was then created with a centrifugal pump, equipped with connection tubes. The inflow tube of the pump conveyed saline from the basin to the pump; the outflow cannula was inserted through the pulmonary artery, across the pulmonary valve, into the right ventricle. The pump was activated to pressurize the right ventricle, thus inducing tricuspid valve regurgitation (TVR). The regurgitant flow through the valve was quantified using a flow-meter. Radiopaque markers were sutured to the head of each papillary muscle and to the tricuspid annulus, in order to trace the geometric changes of the tricuspid valve at increasing pump rates, using fluoroscopy. The efficacy of the bench-model was validated with 10 hearts. Results: The TVR was increased proportionally with the right ventricular pressure (RVP) (TVR = 0.089xRVP - 1.515; R2 = 0.89). The increase in TVR was associated with increases in the annular-to-papillary muscles distance (APML) (TVR = 0.059xAPML - 2.94; R2 = 0.96), of the inter-papillary muscles distance (PMD) (TVR = 0.058xPMD - 8.58; R2 = 0.94), and of the triscuspid annular dilatation (TAD) (TVR = 0.05xTAD - 1.85; R2 = 0.89). Of these parameters, APML was the strongest predictor of TVR. Conclusion: The porcine heart bench model represents a reproducible system to simulate the physiopathology of FTR, and has the potential to serve as a complementary method for the evaluation of new 'in vitro' technologies and therapies for FTR.

Original languageEnglish
Pages (from-to)657-663
Number of pages7
JournalJournal of Heart Valve Disease
Volume20
Issue number6
Publication statusPublished - Nov 2011

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Tricuspid Valve Insufficiency
Swine
Papillary Muscles
Heart Ventricles
Pulmonary Valve
Tricuspid Valve
Fluoroscopy
Ventricular Pressure
Pulmonary Artery
Dilatation

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Maisano, F., Taramasso, M., Guidotti, A., Redaelli, A., Fiore, G. B., Baroni, G., ... Alfieri, O. (2011). Simulation of functional tricuspid regurgitation using an isolated porcine heart model. Journal of Heart Valve Disease, 20(6), 657-663.

Simulation of functional tricuspid regurgitation using an isolated porcine heart model. / Maisano, Francesco; Taramasso, Maurizio; Guidotti, Andrea; Redaelli, Alberto; Fiore, Gianfranco B.; Baroni, Guido; Patete, Paolo; Alfieri, Ottavio.

In: Journal of Heart Valve Disease, Vol. 20, No. 6, 11.2011, p. 657-663.

Research output: Contribution to journalArticle

Maisano, F, Taramasso, M, Guidotti, A, Redaelli, A, Fiore, GB, Baroni, G, Patete, P & Alfieri, O 2011, 'Simulation of functional tricuspid regurgitation using an isolated porcine heart model', Journal of Heart Valve Disease, vol. 20, no. 6, pp. 657-663.
Maisano F, Taramasso M, Guidotti A, Redaelli A, Fiore GB, Baroni G et al. Simulation of functional tricuspid regurgitation using an isolated porcine heart model. Journal of Heart Valve Disease. 2011 Nov;20(6):657-663.
Maisano, Francesco ; Taramasso, Maurizio ; Guidotti, Andrea ; Redaelli, Alberto ; Fiore, Gianfranco B. ; Baroni, Guido ; Patete, Paolo ; Alfieri, Ottavio. / Simulation of functional tricuspid regurgitation using an isolated porcine heart model. In: Journal of Heart Valve Disease. 2011 ; Vol. 20, No. 6. pp. 657-663.
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AU - Patete, Paolo

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