Simultaneous and successive localization of two antigens in the same tissue section using 3,3′-diaminobenzidine and 1-naphthol basic dye

A. Mauro, A. Bulfone, G. Giaccone, D. Schiffer

Research output: Contribution to journalArticle

Abstract

We describe two procedures for the simultaneous and successive localization of two antigens in the same tissue section. In the simultaneous staining procedure, the first antigen was localized using 3,3′-diaminobenzidine (DAB), while the second antigen was stained using the 1-naphthol basic dye (1-NBD) method. The colour of the second antigen depended on the basic dye used, and no mixing of colours was observed when the two antigens were localized in different cells or structures. However, sequential double staining proved to be more convenient for the demonstration of two antigens in the same cell. In this procedure, the first antigen was stained using 1-NBD, and the interesting microscopic fields were photographed. The basic dye was then completely removed, and the second antigen was stained using DAB.

Original languageEnglish
Pages (from-to)123-126
Number of pages4
JournalHistochemistry
Volume86
Issue number2
DOIs
Publication statusPublished - Mar 1986

ASJC Scopus subject areas

  • Anatomy

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