Simultaneous detection of ΔF508, G542X, N1303K, G551D, and 1717-1G→A cystic fibrosis alleles by a multiplex DNA enzyme immunoassay

F. Sangiuolo, P. Macrratesi, A. Mesoraca, A. Botta, A. Cavicchini, G. Novelli, B. Dallapiccola

Research output: Contribution to journalArticlepeer-review

Abstract

We describe the use of a polymerase chain reaction in conjunction with a DNA enzyme immunoassay for the simultaneous detection of five common cystic fibrosis mutations. The method is specific, sensitive, rapid, and proved effective in Guthrie card-based screening of cystic fibrosis mutations.

Original languageEnglish
Pages (from-to)142-145
Number of pages4
JournalInternational Journal of Clinical & Laboratory Research
Volume25
Issue number3
DOIs
Publication statusPublished - Sep 1995

Keywords

  • Cystic fibrosis
  • DNA enzyme immunoassay
  • Polymerase chain reaction
  • Screening

ASJC Scopus subject areas

  • Clinical Biochemistry

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