Simultaneous detection of ΔF508, G542X, N1303K, G551D, and 1717-1G→A cystic fibrosis alleles by a multiplex DNA enzyme immunoassay

F. Sangiuolo; P. Macrratesi; A. Mesoraca; A. Botta; A. Cavicchini; G. Novelli; B. Dallapiccola

doi:10.1007/BF02592555

Simultaneous detection of ΔF508, G542X, N1303K, G551D, and 1717-1G→A cystic fibrosis alleles by a multiplex DNA enzyme immunoassay

F. Sangiuolo, P. Macrratesi, A. Mesoraca, A. Botta, A. Cavicchini, G. Novelli, B. Dallapiccola

Research output: Contribution to journal › Article › peer-review

Abstract

We describe the use of a polymerase chain reaction in conjunction with a DNA enzyme immunoassay for the simultaneous detection of five common cystic fibrosis mutations. The method is specific, sensitive, rapid, and proved effective in Guthrie card-based screening of cystic fibrosis mutations.

Original language	English
Pages (from-to)	142-145
Number of pages	4
Journal	International Journal of Clinical & Laboratory Research
Volume	25
Issue number	3
DOIs	https://doi.org/10.1007/BF02592555
Publication status	Published - Sep 1995

Keywords

Cystic fibrosis
DNA enzyme immunoassay
Polymerase chain reaction
Screening

ASJC Scopus subject areas

Clinical Biochemistry

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10.1007/BF02592555

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Simultaneous detection of ΔF508, G542X, N1303K, G551D, and 1717-1G→A cystic fibrosis alleles by a multiplex DNA enzyme immunoassay. / Sangiuolo, F.; Macrratesi, P.; Mesoraca, A.; Botta, A.; Cavicchini, A.; Novelli, G.; Dallapiccola, B.

In: International Journal of Clinical & Laboratory Research, Vol. 25, No. 3, 09.1995, p. 142-145.

Research output: Contribution to journal › Article › peer-review

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abstract = "We describe the use of a polymerase chain reaction in conjunction with a DNA enzyme immunoassay for the simultaneous detection of five common cystic fibrosis mutations. The method is specific, sensitive, rapid, and proved effective in Guthrie card-based screening of cystic fibrosis mutations.",

keywords = "Cystic fibrosis, DNA enzyme immunoassay, Polymerase chain reaction, Screening",

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AU - Cavicchini, A.

AU - Novelli, G.

AU - Dallapiccola, B.

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Simultaneous detection of ΔF508, G542X, N1303K, G551D, and 1717-1G→A cystic fibrosis alleles by a multiplex DNA enzyme immunoassay

Abstract

Keywords

ASJC Scopus subject areas

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