Abstract
HCV infection is usually diagnosed by means of an enzyme immune assay for the detection of antibody against HCV. The window period between infection and seroconversion remains a dramatic problem in the transfusional and diagnostic setting. In this chapter, we report (i) procedures for assays using two different approaches designed to reduce the window period and (ii) performance in terms of specificity and sensitivity in the detection of both antibody and antigen, and we compare their efficacy with that of commercial assays.
| Original language | English |
|---|---|
| Pages (from-to) | 15-23 |
| Number of pages | 9 |
| Journal | Methods in molecular biology (Clifton, N.J.) |
| Volume | 510 |
| Publication status | Published - 2009 |
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ASJC Scopus subject areas
- Genetics
- Molecular Biology
Cite this
Simultaneous detection of anti-HCV antibody and HCV core antigen. / Ansaldi, Filippo; Icardi, Giancarlo.
In: Methods in molecular biology (Clifton, N.J.), Vol. 510, 2009, p. 15-23.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Simultaneous detection of anti-HCV antibody and HCV core antigen.
AU - Ansaldi, Filippo
AU - Icardi, Giancarlo
PY - 2009
Y1 - 2009
N2 - HCV infection is usually diagnosed by means of an enzyme immune assay for the detection of antibody against HCV. The window period between infection and seroconversion remains a dramatic problem in the transfusional and diagnostic setting. In this chapter, we report (i) procedures for assays using two different approaches designed to reduce the window period and (ii) performance in terms of specificity and sensitivity in the detection of both antibody and antigen, and we compare their efficacy with that of commercial assays.
AB - HCV infection is usually diagnosed by means of an enzyme immune assay for the detection of antibody against HCV. The window period between infection and seroconversion remains a dramatic problem in the transfusional and diagnostic setting. In this chapter, we report (i) procedures for assays using two different approaches designed to reduce the window period and (ii) performance in terms of specificity and sensitivity in the detection of both antibody and antigen, and we compare their efficacy with that of commercial assays.
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UR - http://www.scopus.com/inward/citedby.url?scp=61549135788&partnerID=8YFLogxK
M3 - Article
VL - 510
SP - 15
EP - 23
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
SN - 1064-3745
ER -