Simultaneous detection of fourteen Italian cystic fibrosis mutations in seven exons by reverse dot-blot analysis

M. Rady, E. D'Alcamo, M. Seia, L. Iapichino, M. Ferrari, S. Russo, G. Romeo, A. Maggio

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The increasing number of cystic fibrosis (CF) mutations is a great obstacle to the use of DNA procedures in the detection of gene defects. We describe a fast, cheap and non-radioactive procedure, Reverse dot-blot analysis (RDB), for the simultaneous detection of CF mutations in the Italian population. We used this approach to study seven exons of the CF gene for 14 CF gene defects and were able to characterize 222 of 272 CF chromosomes (80%). The cost of the procedure was $25 per sample analysed.

Original languageEnglish
Pages (from-to)357-360
Number of pages4
JournalMolecular and Cellular Probes
Volume9
Issue number5
DOIs
Publication statusPublished - 1995

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Cystic Fibrosis
Exons
Mutation
Genes
Chromosomes
Costs and Cost Analysis
DNA
Population

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Simultaneous detection of fourteen Italian cystic fibrosis mutations in seven exons by reverse dot-blot analysis. / Rady, M.; D'Alcamo, E.; Seia, M.; Iapichino, L.; Ferrari, M.; Russo, S.; Romeo, G.; Maggio, A.

In: Molecular and Cellular Probes, Vol. 9, No. 5, 1995, p. 357-360.

Research output: Contribution to journalArticle

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