Simultaneous determination of cyclophosphamide, ifosfamide, doxorubicin, epirubicin and daunorubicin in human urine using high-performance liquid chromatography/electrospray ionization tandem mass spectrometry: Bioanalytical method validation

Cristina Sottani, Paola Rinaldi, Emanuela Leoni, Guido Poggi, Cristina Teragni, Angelo Delmonte, Claudio Minoia

Research output: Contribution to journalArticlepeer-review

Abstract

A reversed-phase high-performance liquid chromatography (rp-HPLC) system interfaced with an electrospray ionization (ESI) source coupled to tandem mass spectrometry (MS/MS) was developed and validated for the determination of cyclophosphamide (CP), ifosfamide (IF), daunorubicin (DNR), doxorubicin (DXR), and epirubicin (EPI) in human urine. The analysis of samples containing multiple analytes with a dissimilar range of polarities was carried out using a conventional reversed-phase chromatographic BDS Hypersil C8 column. The analytical run was 15 min. The triple quadrupole mass spectrometer was operated in positive ion mode and multiple reaction monitoring (MRM) was used for drug quantification. The method was validated over a concentration range of 0.2 to 4.0 μg·L-1 for CP, IF, DXR, EPI and 0.15-2.0 μg L -1 for DNR in human urine. The lower limit of quantification (LLOQ) was 0.2 μg·L-1 for CP, IF, EPI and was set at 0.3 and 0.15 μg·L-1 for DXR and DNR, respectively. The relative standard deviations (RSD%) were

Original languageEnglish
Pages (from-to)2645-2659
Number of pages15
JournalRapid Communications in Mass Spectrometry
Volume22
Issue number17
DOIs
Publication statusPublished - Sep 2008

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

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