TY - JOUR
T1 - Simultaneous determination of everolimus and cyclosporine concentrations by HPLC with ultraviolet detection
AU - Baldelli, Sara
AU - Zenoni, Stefania
AU - Merlini, Simona
AU - Perico, Norberto
AU - Cattaneo, Dario
PY - 2006/2
Y1 - 2006/2
N2 - In the clinical practice of organ transplantation everolimus (RAD) is used in combination with cyclosporine (CsA), the most common antirejection agent. Both drugs show a narrow therapeutic window, which requires strict monitoring of their blood concentration. Simple methods for simultaneous measurement of RAD and CsA concentration are needed. As we have recently developed an HPLC-UV assay for RAD determination, we decided to implement it to allow concomitant measurement of CsA. The within- and between-day coefficients of variation of the measurement were less than 12.1% for RAD and 9.8% for CsA. The within- and between-day inaccuracy of quality control samples were less than 9.7% for RAD and less than 4.9% for CsA. The method was found accurate and precise and useful for simultaneous therapeutic monitoring of the two drugs.
AB - In the clinical practice of organ transplantation everolimus (RAD) is used in combination with cyclosporine (CsA), the most common antirejection agent. Both drugs show a narrow therapeutic window, which requires strict monitoring of their blood concentration. Simple methods for simultaneous measurement of RAD and CsA concentration are needed. As we have recently developed an HPLC-UV assay for RAD determination, we decided to implement it to allow concomitant measurement of CsA. The within- and between-day coefficients of variation of the measurement were less than 12.1% for RAD and 9.8% for CsA. The within- and between-day inaccuracy of quality control samples were less than 9.7% for RAD and less than 4.9% for CsA. The method was found accurate and precise and useful for simultaneous therapeutic monitoring of the two drugs.
KW - Cyclosporine
KW - Everolimus
KW - HPLC
KW - Therapeutic drug monitoring
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U2 - 10.1016/j.cca.2005.07.019
DO - 10.1016/j.cca.2005.07.019
M3 - Article
C2 - 16153624
AN - SCOPUS:31044444888
VL - 364
SP - 354
EP - 358
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
SN - 0009-8981
IS - 1-2
ER -