Abstract
A simple, accurate and sensitive high-performance liquid chromatographic method with UV detection was carried out to measure simultaneously plasma and urine concentrations of both p-aminohippuric acid and inulin. Following a simplified acid hydrolysis of the sample, the separation was carried out in 4 min using a C18 reversed-phase column with a flow-rate of 1 ml/min, and monitoring the absorbance at 280 nm. Within the investigated concentration ranges of inulin (0.1-3.2 mg/ml) and p-aminohippuric acid (0.0097-0.3 mg/ml), good linearity (r>0.99) was obtained. Within-run RSD ranged from 2.9 to 6.1% and between-run RSD ranged from 6.4 to 10%. Analytical recoveries were 101-112%, with little differences between plasma and urine samples. The detection limit was 1 μg/ml for all the analytes studied. This method might be ideal for renal function studies where a rapid and reproducible assessment of both renal glomerular filtration rate and blood flow-rate is required.
| Original language | English |
|---|---|
| Pages (from-to) | 187-191 |
| Number of pages | 5 |
| Journal | Journal of Chromatography B: Biomedical Sciences and Applications |
| Volume | 751 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Feb 10 2001 |
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Keywords
- Inulin
- p-Aminohippuric acid
ASJC Scopus subject areas
- Chemistry(all)
Cite this
Simultaneous determination of inulin and p-aminohippuric acid in plasma and urine by reversed-phase high-performance liquid chromatography. / Pastore, Anna; Bernardini, Sergio; Dello Strologo, Luca; Rizzoni, Gianfranco; Cortese, Claudio; Federici, Giorgio.
In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 751, No. 1, 10.02.2001, p. 187-191.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Simultaneous determination of inulin and p-aminohippuric acid in plasma and urine by reversed-phase high-performance liquid chromatography
AU - Pastore, Anna
AU - Bernardini, Sergio
AU - Dello Strologo, Luca
AU - Rizzoni, Gianfranco
AU - Cortese, Claudio
AU - Federici, Giorgio
PY - 2001/2/10
Y1 - 2001/2/10
N2 - A simple, accurate and sensitive high-performance liquid chromatographic method with UV detection was carried out to measure simultaneously plasma and urine concentrations of both p-aminohippuric acid and inulin. Following a simplified acid hydrolysis of the sample, the separation was carried out in 4 min using a C18 reversed-phase column with a flow-rate of 1 ml/min, and monitoring the absorbance at 280 nm. Within the investigated concentration ranges of inulin (0.1-3.2 mg/ml) and p-aminohippuric acid (0.0097-0.3 mg/ml), good linearity (r>0.99) was obtained. Within-run RSD ranged from 2.9 to 6.1% and between-run RSD ranged from 6.4 to 10%. Analytical recoveries were 101-112%, with little differences between plasma and urine samples. The detection limit was 1 μg/ml for all the analytes studied. This method might be ideal for renal function studies where a rapid and reproducible assessment of both renal glomerular filtration rate and blood flow-rate is required.
AB - A simple, accurate and sensitive high-performance liquid chromatographic method with UV detection was carried out to measure simultaneously plasma and urine concentrations of both p-aminohippuric acid and inulin. Following a simplified acid hydrolysis of the sample, the separation was carried out in 4 min using a C18 reversed-phase column with a flow-rate of 1 ml/min, and monitoring the absorbance at 280 nm. Within the investigated concentration ranges of inulin (0.1-3.2 mg/ml) and p-aminohippuric acid (0.0097-0.3 mg/ml), good linearity (r>0.99) was obtained. Within-run RSD ranged from 2.9 to 6.1% and between-run RSD ranged from 6.4 to 10%. Analytical recoveries were 101-112%, with little differences between plasma and urine samples. The detection limit was 1 μg/ml for all the analytes studied. This method might be ideal for renal function studies where a rapid and reproducible assessment of both renal glomerular filtration rate and blood flow-rate is required.
KW - Inulin
KW - p-Aminohippuric acid
UR - http://www.scopus.com/inward/record.url?scp=0035835421&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035835421&partnerID=8YFLogxK
U2 - 10.1016/S0378-4347(00)00444-8
DO - 10.1016/S0378-4347(00)00444-8
M3 - Article
VL - 751
SP - 187
EP - 191
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
SN - 1387-2273
IS - 1
ER -