Simultaneous determination of inulin and p-aminohippuric acid in plasma and urine by reversed-phase high-performance liquid chromatography

Anna Pastore, Sergio Bernardini, Luca Dello Strologo, Gianfranco Rizzoni, Claudio Cortese, Giorgio Federici

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

A simple, accurate and sensitive high-performance liquid chromatographic method with UV detection was carried out to measure simultaneously plasma and urine concentrations of both p-aminohippuric acid and inulin. Following a simplified acid hydrolysis of the sample, the separation was carried out in 4 min using a C18 reversed-phase column with a flow-rate of 1 ml/min, and monitoring the absorbance at 280 nm. Within the investigated concentration ranges of inulin (0.1-3.2 mg/ml) and p-aminohippuric acid (0.0097-0.3 mg/ml), good linearity (r>0.99) was obtained. Within-run RSD ranged from 2.9 to 6.1% and between-run RSD ranged from 6.4 to 10%. Analytical recoveries were 101-112%, with little differences between plasma and urine samples. The detection limit was 1 μg/ml for all the analytes studied. This method might be ideal for renal function studies where a rapid and reproducible assessment of both renal glomerular filtration rate and blood flow-rate is required.

Original languageEnglish
Pages (from-to)187-191
Number of pages5
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume751
Issue number1
DOIs
Publication statusPublished - Feb 10 2001

Fingerprint

p-Aminohippuric Acid
Inulin
High performance liquid chromatography
Flow rate
Plasmas
Hydrolysis
Blood
Recovery
Acids
Monitoring
Liquids

Keywords

  • Inulin
  • p-Aminohippuric acid

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Simultaneous determination of inulin and p-aminohippuric acid in plasma and urine by reversed-phase high-performance liquid chromatography. / Pastore, Anna; Bernardini, Sergio; Dello Strologo, Luca; Rizzoni, Gianfranco; Cortese, Claudio; Federici, Giorgio.

In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 751, No. 1, 10.02.2001, p. 187-191.

Research output: Contribution to journalArticle

@article{5f1be530c12b4b78a7b494e9d95a4335,
title = "Simultaneous determination of inulin and p-aminohippuric acid in plasma and urine by reversed-phase high-performance liquid chromatography",
abstract = "A simple, accurate and sensitive high-performance liquid chromatographic method with UV detection was carried out to measure simultaneously plasma and urine concentrations of both p-aminohippuric acid and inulin. Following a simplified acid hydrolysis of the sample, the separation was carried out in 4 min using a C18 reversed-phase column with a flow-rate of 1 ml/min, and monitoring the absorbance at 280 nm. Within the investigated concentration ranges of inulin (0.1-3.2 mg/ml) and p-aminohippuric acid (0.0097-0.3 mg/ml), good linearity (r>0.99) was obtained. Within-run RSD ranged from 2.9 to 6.1{\%} and between-run RSD ranged from 6.4 to 10{\%}. Analytical recoveries were 101-112{\%}, with little differences between plasma and urine samples. The detection limit was 1 μg/ml for all the analytes studied. This method might be ideal for renal function studies where a rapid and reproducible assessment of both renal glomerular filtration rate and blood flow-rate is required.",
keywords = "Inulin, p-Aminohippuric acid",
author = "Anna Pastore and Sergio Bernardini and {Dello Strologo}, Luca and Gianfranco Rizzoni and Claudio Cortese and Giorgio Federici",
year = "2001",
month = "2",
day = "10",
doi = "10.1016/S0378-4347(00)00444-8",
language = "English",
volume = "751",
pages = "187--191",
journal = "Journal of Chromatography B: Biomedical Sciences and Applications",
issn = "1387-2273",
publisher = "Elsevier BV",
number = "1",

}

TY - JOUR

T1 - Simultaneous determination of inulin and p-aminohippuric acid in plasma and urine by reversed-phase high-performance liquid chromatography

AU - Pastore, Anna

AU - Bernardini, Sergio

AU - Dello Strologo, Luca

AU - Rizzoni, Gianfranco

AU - Cortese, Claudio

AU - Federici, Giorgio

PY - 2001/2/10

Y1 - 2001/2/10

N2 - A simple, accurate and sensitive high-performance liquid chromatographic method with UV detection was carried out to measure simultaneously plasma and urine concentrations of both p-aminohippuric acid and inulin. Following a simplified acid hydrolysis of the sample, the separation was carried out in 4 min using a C18 reversed-phase column with a flow-rate of 1 ml/min, and monitoring the absorbance at 280 nm. Within the investigated concentration ranges of inulin (0.1-3.2 mg/ml) and p-aminohippuric acid (0.0097-0.3 mg/ml), good linearity (r>0.99) was obtained. Within-run RSD ranged from 2.9 to 6.1% and between-run RSD ranged from 6.4 to 10%. Analytical recoveries were 101-112%, with little differences between plasma and urine samples. The detection limit was 1 μg/ml for all the analytes studied. This method might be ideal for renal function studies where a rapid and reproducible assessment of both renal glomerular filtration rate and blood flow-rate is required.

AB - A simple, accurate and sensitive high-performance liquid chromatographic method with UV detection was carried out to measure simultaneously plasma and urine concentrations of both p-aminohippuric acid and inulin. Following a simplified acid hydrolysis of the sample, the separation was carried out in 4 min using a C18 reversed-phase column with a flow-rate of 1 ml/min, and monitoring the absorbance at 280 nm. Within the investigated concentration ranges of inulin (0.1-3.2 mg/ml) and p-aminohippuric acid (0.0097-0.3 mg/ml), good linearity (r>0.99) was obtained. Within-run RSD ranged from 2.9 to 6.1% and between-run RSD ranged from 6.4 to 10%. Analytical recoveries were 101-112%, with little differences between plasma and urine samples. The detection limit was 1 μg/ml for all the analytes studied. This method might be ideal for renal function studies where a rapid and reproducible assessment of both renal glomerular filtration rate and blood flow-rate is required.

KW - Inulin

KW - p-Aminohippuric acid

UR - http://www.scopus.com/inward/record.url?scp=0035835421&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035835421&partnerID=8YFLogxK

U2 - 10.1016/S0378-4347(00)00444-8

DO - 10.1016/S0378-4347(00)00444-8

M3 - Article

VL - 751

SP - 187

EP - 191

JO - Journal of Chromatography B: Biomedical Sciences and Applications

JF - Journal of Chromatography B: Biomedical Sciences and Applications

SN - 1387-2273

IS - 1

ER -