Simultaneous determination of inulin and p-aminohippuric acid in plasma and urine by reversed-phase high-performance liquid chromatography

Anna Pastore, Sergio Bernardini, Luca Dello Strologo, Gianfranco Rizzoni, Claudio Cortese, Giorgio Federici

Research output: Contribution to journalArticle

Abstract

A simple, accurate and sensitive high-performance liquid chromatographic method with UV detection was carried out to measure simultaneously plasma and urine concentrations of both p-aminohippuric acid and inulin. Following a simplified acid hydrolysis of the sample, the separation was carried out in 4 min using a C18 reversed-phase column with a flow-rate of 1 ml/min, and monitoring the absorbance at 280 nm. Within the investigated concentration ranges of inulin (0.1-3.2 mg/ml) and p-aminohippuric acid (0.0097-0.3 mg/ml), good linearity (r>0.99) was obtained. Within-run RSD ranged from 2.9 to 6.1% and between-run RSD ranged from 6.4 to 10%. Analytical recoveries were 101-112%, with little differences between plasma and urine samples. The detection limit was 1 μg/ml for all the analytes studied. This method might be ideal for renal function studies where a rapid and reproducible assessment of both renal glomerular filtration rate and blood flow-rate is required.

Original languageEnglish
Pages (from-to)187-191
Number of pages5
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume751
Issue number1
DOIs
Publication statusPublished - Feb 10 2001

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Keywords

  • Inulin
  • p-Aminohippuric acid

ASJC Scopus subject areas

  • Chemistry(all)

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