Simultaneous measurement of phosphatidylglycerol and disaturated- phosphatidylcholine palmitate kinetics from alveolar surfactant. Study in infants with stable isotope tracer, coupled with isotope ratio mass spectrometry

Luca Vedovelli, Aldo Baritussio, Virgilio Paolo Carnielli, Manuela Simonato, Pietro Giusti, Paola Elisa Cogo

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Disaturated-phosphatidylcholine (DSPC) and phosphatidylglycerol (PG) are respectively the first and the third most abundant phospholipid in human alveolar surfactant. Their concentration decreases in airway surfactant of adults and infants with respiratory distress syndrome and cystic fibrosis. In this study, we used mass spectrometry (IRMS) to investigate the turnover of DSPC and PG in tracheal aspirates (TA) obtained from infants with normal or diseased lungs. We studied eight infants requiring mechanical ventilation: two with no lung disease, four with diaphragmatic hernia, one with ATP-binding cassette sub-family A member 3 heterozygote mutation and one with sepsis. Patients received deuterated water for 48 h as metabolic precursors of palmitate-DSPC and palmitate-PG. Serial TAs were obtained every 6 h for five days or until extubation. DSPC and PG were isolated from TA by column and high-performance thin layer chromatography. Deuterium enrichments of palmitate-DSPC and PG residues were measured by IRMS coupled with a gas chromatographer. Median secretion time (ST), peak time (PT) and fractional synthesis rate (FSR) were 3.7 [0.9- 13.4] h, 71.0 [52.2 - 85.2] h and 6.6 [6.3 - 11.1] %/day for DSPC and 19.3 [6.4 - 22.8] h, 49.0 [33.0 - 52.5] h and 5.8 [4.8 - 10.9] %/day for PG. This study shows that it is feasible to use deuterium derived from body water to trace simultaneously airway surfactant DSPC and PG in humans. When compared within the same patient, DSPC and PG had similar fractional synthesis rates, but PG had a shorter PT, suggesting differences in the life cycle of these essential surfactant components.

Original languageEnglish
Pages (from-to)986-992
Number of pages7
JournalJournal of Mass Spectrometry
Volume46
Issue number10
DOIs
Publication statusPublished - Oct 2011

Fingerprint

Radioactive tracers
Phosphatidylglycerols
Palmitates
isotope ratios
Surface-Active Agents
Isotopes
tracers
Mass spectrometry
Mass Spectrometry
Surface active agents
mass spectroscopy
isotopes
surfactants
Kinetics
kinetics
Deuterium
lungs
deuterium
cystic fibrosis
thin layer chromatography

Keywords

  • kinetics
  • lung surfactant
  • palmitate
  • phophatidylglycerol
  • phosphatidylcholine
  • phospholipids
  • RDS

ASJC Scopus subject areas

  • Spectroscopy

Cite this

Simultaneous measurement of phosphatidylglycerol and disaturated- phosphatidylcholine palmitate kinetics from alveolar surfactant. Study in infants with stable isotope tracer, coupled with isotope ratio mass spectrometry. / Vedovelli, Luca; Baritussio, Aldo; Carnielli, Virgilio Paolo; Simonato, Manuela; Giusti, Pietro; Cogo, Paola Elisa.

In: Journal of Mass Spectrometry, Vol. 46, No. 10, 10.2011, p. 986-992.

Research output: Contribution to journalArticle

Vedovelli, Luca ; Baritussio, Aldo ; Carnielli, Virgilio Paolo ; Simonato, Manuela ; Giusti, Pietro ; Cogo, Paola Elisa. / Simultaneous measurement of phosphatidylglycerol and disaturated- phosphatidylcholine palmitate kinetics from alveolar surfactant. Study in infants with stable isotope tracer, coupled with isotope ratio mass spectrometry. In: Journal of Mass Spectrometry. 2011 ; Vol. 46, No. 10. pp. 986-992.
@article{7a125c9b96a0432ea8e1553cfed14e3d,
title = "Simultaneous measurement of phosphatidylglycerol and disaturated- phosphatidylcholine palmitate kinetics from alveolar surfactant. Study in infants with stable isotope tracer, coupled with isotope ratio mass spectrometry",
abstract = "Disaturated-phosphatidylcholine (DSPC) and phosphatidylglycerol (PG) are respectively the first and the third most abundant phospholipid in human alveolar surfactant. Their concentration decreases in airway surfactant of adults and infants with respiratory distress syndrome and cystic fibrosis. In this study, we used mass spectrometry (IRMS) to investigate the turnover of DSPC and PG in tracheal aspirates (TA) obtained from infants with normal or diseased lungs. We studied eight infants requiring mechanical ventilation: two with no lung disease, four with diaphragmatic hernia, one with ATP-binding cassette sub-family A member 3 heterozygote mutation and one with sepsis. Patients received deuterated water for 48 h as metabolic precursors of palmitate-DSPC and palmitate-PG. Serial TAs were obtained every 6 h for five days or until extubation. DSPC and PG were isolated from TA by column and high-performance thin layer chromatography. Deuterium enrichments of palmitate-DSPC and PG residues were measured by IRMS coupled with a gas chromatographer. Median secretion time (ST), peak time (PT) and fractional synthesis rate (FSR) were 3.7 [0.9- 13.4] h, 71.0 [52.2 - 85.2] h and 6.6 [6.3 - 11.1] {\%}/day for DSPC and 19.3 [6.4 - 22.8] h, 49.0 [33.0 - 52.5] h and 5.8 [4.8 - 10.9] {\%}/day for PG. This study shows that it is feasible to use deuterium derived from body water to trace simultaneously airway surfactant DSPC and PG in humans. When compared within the same patient, DSPC and PG had similar fractional synthesis rates, but PG had a shorter PT, suggesting differences in the life cycle of these essential surfactant components.",
keywords = "kinetics, lung surfactant, palmitate, phophatidylglycerol, phosphatidylcholine, phospholipids, RDS",
author = "Luca Vedovelli and Aldo Baritussio and Carnielli, {Virgilio Paolo} and Manuela Simonato and Pietro Giusti and Cogo, {Paola Elisa}",
year = "2011",
month = "10",
doi = "10.1002/jms.1979",
language = "English",
volume = "46",
pages = "986--992",
journal = "Biomedical Mass Spectrometry",
issn = "1076-5174",
publisher = "John Wiley and Sons Ltd",
number = "10",

}

TY - JOUR

T1 - Simultaneous measurement of phosphatidylglycerol and disaturated- phosphatidylcholine palmitate kinetics from alveolar surfactant. Study in infants with stable isotope tracer, coupled with isotope ratio mass spectrometry

AU - Vedovelli, Luca

AU - Baritussio, Aldo

AU - Carnielli, Virgilio Paolo

AU - Simonato, Manuela

AU - Giusti, Pietro

AU - Cogo, Paola Elisa

PY - 2011/10

Y1 - 2011/10

N2 - Disaturated-phosphatidylcholine (DSPC) and phosphatidylglycerol (PG) are respectively the first and the third most abundant phospholipid in human alveolar surfactant. Their concentration decreases in airway surfactant of adults and infants with respiratory distress syndrome and cystic fibrosis. In this study, we used mass spectrometry (IRMS) to investigate the turnover of DSPC and PG in tracheal aspirates (TA) obtained from infants with normal or diseased lungs. We studied eight infants requiring mechanical ventilation: two with no lung disease, four with diaphragmatic hernia, one with ATP-binding cassette sub-family A member 3 heterozygote mutation and one with sepsis. Patients received deuterated water for 48 h as metabolic precursors of palmitate-DSPC and palmitate-PG. Serial TAs were obtained every 6 h for five days or until extubation. DSPC and PG were isolated from TA by column and high-performance thin layer chromatography. Deuterium enrichments of palmitate-DSPC and PG residues were measured by IRMS coupled with a gas chromatographer. Median secretion time (ST), peak time (PT) and fractional synthesis rate (FSR) were 3.7 [0.9- 13.4] h, 71.0 [52.2 - 85.2] h and 6.6 [6.3 - 11.1] %/day for DSPC and 19.3 [6.4 - 22.8] h, 49.0 [33.0 - 52.5] h and 5.8 [4.8 - 10.9] %/day for PG. This study shows that it is feasible to use deuterium derived from body water to trace simultaneously airway surfactant DSPC and PG in humans. When compared within the same patient, DSPC and PG had similar fractional synthesis rates, but PG had a shorter PT, suggesting differences in the life cycle of these essential surfactant components.

AB - Disaturated-phosphatidylcholine (DSPC) and phosphatidylglycerol (PG) are respectively the first and the third most abundant phospholipid in human alveolar surfactant. Their concentration decreases in airway surfactant of adults and infants with respiratory distress syndrome and cystic fibrosis. In this study, we used mass spectrometry (IRMS) to investigate the turnover of DSPC and PG in tracheal aspirates (TA) obtained from infants with normal or diseased lungs. We studied eight infants requiring mechanical ventilation: two with no lung disease, four with diaphragmatic hernia, one with ATP-binding cassette sub-family A member 3 heterozygote mutation and one with sepsis. Patients received deuterated water for 48 h as metabolic precursors of palmitate-DSPC and palmitate-PG. Serial TAs were obtained every 6 h for five days or until extubation. DSPC and PG were isolated from TA by column and high-performance thin layer chromatography. Deuterium enrichments of palmitate-DSPC and PG residues were measured by IRMS coupled with a gas chromatographer. Median secretion time (ST), peak time (PT) and fractional synthesis rate (FSR) were 3.7 [0.9- 13.4] h, 71.0 [52.2 - 85.2] h and 6.6 [6.3 - 11.1] %/day for DSPC and 19.3 [6.4 - 22.8] h, 49.0 [33.0 - 52.5] h and 5.8 [4.8 - 10.9] %/day for PG. This study shows that it is feasible to use deuterium derived from body water to trace simultaneously airway surfactant DSPC and PG in humans. When compared within the same patient, DSPC and PG had similar fractional synthesis rates, but PG had a shorter PT, suggesting differences in the life cycle of these essential surfactant components.

KW - kinetics

KW - lung surfactant

KW - palmitate

KW - phophatidylglycerol

KW - phosphatidylcholine

KW - phospholipids

KW - RDS

UR - http://www.scopus.com/inward/record.url?scp=80054830131&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80054830131&partnerID=8YFLogxK

U2 - 10.1002/jms.1979

DO - 10.1002/jms.1979

M3 - Article

VL - 46

SP - 986

EP - 992

JO - Biomedical Mass Spectrometry

JF - Biomedical Mass Spectrometry

SN - 1076-5174

IS - 10

ER -