Simultaneous quantification of 8-iso-prostaglandin-F2α and 11-dehydro thromboxane B2 in human urine by liquid chromatography-tandem mass spectrometry

Viviana Cavalca, Fabiana Minardi, Samuele Scurati, Federico Guidugli, Isabella Squellerio, Fabrizio Veglia, Luca Dainese, Anna Guarino, Elena Tremoli, Donatella Caruso

Research output: Contribution to journalArticle

Abstract

Both F2-isoprostanes (8-iso-PGF), a well-known marker of oxidative stress, and thromboxanes A2 (TXA2) are involved in atherosclerosis through LDL oxidation and platelet activation. Different aspects of the pathology can be described by 8-iso-PGF and TXA2 so it is important to determine both their concentrations to monitor the disease progression and/or therapy effects. We developed a simple and sensitive method based on liquid chromatography-tandem mass spectrometry, using electrospray ionization in negative-ion mode, for the simultaneous measurement of the concentration of 8-iso-PGF and 11-dehydro thromboxane B2 (11-DH-TXB2), a TXA2 metabolite. This method was applied to analyze urine samples collected overnight from 15 atherosclerotic patients, with documented carotid artery sclerosis (CAS), and from 20 controls. The detection limit was 0.097 pg/μL for 8-iso-PGF and 0.375 pg/μL for 11-DH-TXB2, with a linear range of 0.78-25 pg/μL; the inter- and intraday imprecision was 2α and 11-DH-TXB2 in human urine samples in order to evaluate oxidative stress and platelet aggregation.

Original languageEnglish
Pages (from-to)168-174
Number of pages7
JournalAnalytical Biochemistry
Volume397
Issue number2
DOIs
Publication statusPublished - Feb 15 2010

Keywords

  • Carotid artery stenosis
  • Liquid chromatography-tandem mass spectrometry
  • Oxidative stress
  • Platelet aggregation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology

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