Single-dose intraperitoneal magainins improve survival in a gram-negative-pathogen septic shock rat model

O. Cirioni, A. Giacometti, R. Ghiselli, F. Mocchegiani, A. Fineo, F. Orlando, M. S. Del Prete, M. Rocchi, V. Saba, G. Scalise

Research output: Contribution to journalArticle

Abstract

The therapeutic etticacies of three polycationic peptides selected among the class of the magainins (magainin I, magainin II, and magainin II amide), alone and combined with piperacillin, were investigated in a rat model of septic shock. Rats were given an intraperitoneal injection of 2 × 1010 CFU of Escherichia coli and randomized to receive intraperitoneally isotonic sodium chloride solution, 60 mg of piperacillin per kg of body weight, and 1 mg of each magainin per kg alone and combined with 60 mg of piperacillin per kg. The main outcome measures were bacterial growth in abdominal exudate and plasma, endotoxin and tumor necrosis factor alpha (TNF-α) concentrations in plasma, and lethality. Treatments with the magainins achieved significant reductions of bacterial growth and plasma endotoxin and TNF-α concentrations. In general, treatments with the combinations of magainins and piperaciilin demonstrated the highest efficacies.

Original languageEnglish
Pages (from-to)101-104
Number of pages4
JournalAntimicrobial Agents and Chemotherapy
Volume46
Issue number1
DOIs
Publication statusPublished - 2002

ASJC Scopus subject areas

  • Pharmacology (medical)

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    Cirioni, O., Giacometti, A., Ghiselli, R., Mocchegiani, F., Fineo, A., Orlando, F., Del Prete, M. S., Rocchi, M., Saba, V., & Scalise, G. (2002). Single-dose intraperitoneal magainins improve survival in a gram-negative-pathogen septic shock rat model. Antimicrobial Agents and Chemotherapy, 46(1), 101-104. https://doi.org/10.1128/AAC.46.1.101-104.2002