Single living cell encapsulation in nano-organized polyelectrolyte shells

Alberto Diaspro; Daniela Silvano; Silke Krol; Ornella Cavalleri; Alessandra Gliozzi

doi:10.1021/la025646e

Single living cell encapsulation in nano-organized polyelectrolyte shells

Alberto Diaspro, Daniela Silvano, Silke Krol, Ornella Cavalleri, Alessandra Gliozzi

IRCCS Giovanni Paolo II

Research output: Contribution to journal › Article › peer-review

Abstract

Single living yeast cells have been encapsulated by the alternate adsorption of oppositely charged polyelectrolytes. Exploiting fluorescence techniques, we provide evidence of the shell and cell integrity after the coating procedure. The most relevant result, however, is that, after encapsulation, cells preserve their metabolic activities and they are still able to divide. These hybrid polyelectrolyte cells can provide a cheap model system in a wide range of biophysical and biotechnological applications, thanks to the tunable properties of the polyelectrolyte shell.

Original language	English
Pages (from-to)	5047-5050
Number of pages	4
Journal	Langmuir
Volume	18
Issue number	13
DOIs	https://doi.org/10.1021/la025646e
Publication status	Published - Jun 25 2002

ASJC Scopus subject areas

Physical and Theoretical Chemistry
Colloid and Surface Chemistry

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AU - Diaspro, Alberto

AU - Silvano, Daniela

AU - Krol, Silke

AU - Cavalleri, Ornella

AU - Gliozzi, Alessandra

PY - 2002/6/25

Y1 - 2002/6/25

N2 - Single living yeast cells have been encapsulated by the alternate adsorption of oppositely charged polyelectrolytes. Exploiting fluorescence techniques, we provide evidence of the shell and cell integrity after the coating procedure. The most relevant result, however, is that, after encapsulation, cells preserve their metabolic activities and they are still able to divide. These hybrid polyelectrolyte cells can provide a cheap model system in a wide range of biophysical and biotechnological applications, thanks to the tunable properties of the polyelectrolyte shell.

AB - Single living yeast cells have been encapsulated by the alternate adsorption of oppositely charged polyelectrolytes. Exploiting fluorescence techniques, we provide evidence of the shell and cell integrity after the coating procedure. The most relevant result, however, is that, after encapsulation, cells preserve their metabolic activities and they are still able to divide. These hybrid polyelectrolyte cells can provide a cheap model system in a wide range of biophysical and biotechnological applications, thanks to the tunable properties of the polyelectrolyte shell.

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Single living cell encapsulation in nano-organized polyelectrolyte shells

Abstract

ASJC Scopus subject areas

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