Single severe traumatic brain injury produces progressive pathology with ongoing contralateral white matter damage one year after injury

Francesca Pischiutta, Edoardo Micotti, Jennifer R. Hay, Ines Marongiu, Eliana Sammali, Daniele Tolomeo, Gloria Vegliante, Nino Stocchetti, Gianluigi Forloni, Maria Grazia De Simoni, William Stewart, Elisa R. Zanier

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

There is increasing recognition that traumatic brain injury (TBI) may initiate long-term neurodegenerative processes, particularly chronic traumatic encephalopathy. However, insight into the mechanisms transforming an initial biomechanical injury into a neurodegenerative process remain elusive, partly as a consequence of the paucity of informative pre-clinical models. This study shows the functional, whole brain imaging and neuropathological consequences at up to one year survival from single severe TBI by controlled cortical impact in mice. TBI mice displayed persistent sensorimotor and cognitive deficits. Longitudinal T2 weighted magnetic resonance imaging (MRI) showed progressive ipsilateral (il) cortical, hippocampal and striatal volume loss, with diffusion tensor imaging demonstrating decreased fractional anisotropy (FA) at up to one year in the il-corpus callosum (CC: − 30%) and external capsule (EC: − 21%). Parallel neuropathological studies indicated reduction in neuronal density, with evidence of microgliosis and astrogliosis in the il-cortex, with further evidence of microgliosis and astrogliosis in the il-thalamus. One year after TBI there was also a decrease in FA in the contralateral (cl) CC (− 17%) and EC (− 13%), corresponding to histopathological evidence of white matter loss (cl-CC: − 68%; cl-EC: − 30%) associated with ongoing microgliosis and astrogliosis. These findings indicate that a single severe TBI induces bilateral, long-term and progressive neuropathology at up to one year after injury. These observations support this model as a suitable platform for exploring the mechanistic link between acute brain injury and late and persistent neurodegeneration.

Original languageEnglish
Pages (from-to)167-178
Number of pages12
JournalExperimental Neurology
Volume300
DOIs
Publication statusPublished - Feb 1 2018

Fingerprint

Pathology
Wounds and Injuries
Anisotropy
Corpus Striatum
Functional Neuroimaging
Diffusion Tensor Imaging
Corpus Callosum
Thalamus
Brain Injuries
Magnetic Resonance Imaging
White Matter
Traumatic Brain Injury

Keywords

  • Inflammation
  • Long-term outcome
  • Neurodegeneration
  • Neuroimaging
  • Traumatic brain injury
  • White matter damage

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience

Cite this

Single severe traumatic brain injury produces progressive pathology with ongoing contralateral white matter damage one year after injury. / Pischiutta, Francesca; Micotti, Edoardo; Hay, Jennifer R.; Marongiu, Ines; Sammali, Eliana; Tolomeo, Daniele; Vegliante, Gloria; Stocchetti, Nino; Forloni, Gianluigi; De Simoni, Maria Grazia; Stewart, William; Zanier, Elisa R.

In: Experimental Neurology, Vol. 300, 01.02.2018, p. 167-178.

Research output: Contribution to journalArticle

Pischiutta, Francesca ; Micotti, Edoardo ; Hay, Jennifer R. ; Marongiu, Ines ; Sammali, Eliana ; Tolomeo, Daniele ; Vegliante, Gloria ; Stocchetti, Nino ; Forloni, Gianluigi ; De Simoni, Maria Grazia ; Stewart, William ; Zanier, Elisa R. / Single severe traumatic brain injury produces progressive pathology with ongoing contralateral white matter damage one year after injury. In: Experimental Neurology. 2018 ; Vol. 300. pp. 167-178.
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