Single-step affinity purification for fungal proteomics

Hui Lin Liu, Aysha H. Osmani, Leena Ukil, Sunghun Son, Sarine Markossian, Kuo Fang Shen, Meera Govindaraghavan, Archana Varadaraj, Shahr B. Hashmi, Colin P. de Souza, Stephen A. Osmani

Research output: Contribution to journalArticle

Abstract

A single-step protein affinity purification protocol using Aspergillus nidulans is described. Detailed protocols for cell breakage, affinity purification, and depending on the application, methods for protein release from affinity beads are provided. Examples defining the utility of the approaches, which should be widely applicable, are included.

Original languageEnglish
Pages (from-to)831-833
Number of pages3
JournalEukaryotic Cell
Volume9
Issue number5
DOIs
Publication statusPublished - May 2010

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ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Liu, H. L., Osmani, A. H., Ukil, L., Son, S., Markossian, S., Shen, K. F., Govindaraghavan, M., Varadaraj, A., Hashmi, S. B., de Souza, C. P., & Osmani, S. A. (2010). Single-step affinity purification for fungal proteomics. Eukaryotic Cell, 9(5), 831-833. https://doi.org/10.1128/EC.00032-10