Single step determination of PCB 126 and 153 in rat tissues by using solid phase microextraction/gas chromatography-mass spectrometry: Comparison with solid phase extraction and liquid/liquid extraction

Diana Poli, Andrea Caglieri, Matteo Goldoni, Anna F. Castoldi, Teresa Coccini, Elisa Roda, Annabella Vitalone, Sandra Ceccatelli, Antonio Mutti

Research output: Contribution to journalArticlepeer-review

Abstract

A simple and reliable solid phase microextraction/gas chromatography-mass spectrometry (SPME/GC-MS) method was developed for the single-step determination of PCBs 126 and 153 in rat brain and serum, using liquid/liquid and solid phase extraction (SPE) as reference techniques. The multi-factor categorical experimental design used to study simultaneously the main parameters and their interactions affecting the efficiency of the method, showed that the use of an 85 μm PA exposed at 100 °C for 40 min was the optimum sampling condition for both PCBs. SPME was then validated by studying its linear dynamic (over two orders of magnitude), limits of detection (brain: 2 ng/g, serum: 0.2 ng/g) and analytical precision that was within 9% for SPME in both brain and serum. Finally, the method was used to determine the brain and blood target dose in mothers and pups after oral exposure of the mothers.

Original languageEnglish
Pages (from-to)773-783
Number of pages11
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume877
Issue number8-9
DOIs
Publication statusPublished - Mar 15 2009

Keywords

  • Brain and serum tissues
  • Gas chromatography-mass spectrometry
  • Polychlorinated biphenyls
  • Solid phase microextraction

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Cell Biology
  • Clinical Biochemistry

Fingerprint Dive into the research topics of 'Single step determination of PCB 126 and 153 in rat tissues by using solid phase microextraction/gas chromatography-mass spectrometry: Comparison with solid phase extraction and liquid/liquid extraction'. Together they form a unique fingerprint.

Cite this