Abstract
High-performance membrane chromatography (HPMC) and HPLC hydroxyapatite chromatography were compared for their efficiency in purifying immunotoxins (ITs) containing the ribosome-inactivating protein clavin, which is characterized by a high anionic charge and a low molecular mass. Both methods efficiently removed unreacted clavin from the conjugate crude mixture, but only the cation-exchange HPMC allowed efficient single-step separation of the unreacted monoclonal antibody (mAb) from ITs obtained by different coupling procedures. Copyright (C) 1999 Elsevier Science B.V.
| Original language | English |
|---|---|
| Pages (from-to) | 329-335 |
| Number of pages | 7 |
| Journal | Journal of Chromatography A |
| Volume | 830 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Jan 15 1999 |
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Keywords
- Clavin
- Immunotoxins
- Membranes
- Monoclonal antibodies
- Proteins
- Toxins
ASJC Scopus subject areas
- Analytical Chemistry
Cite this
Single-step purification of immunotoxins containing a high ionic charge ribosome inactivating protein clavin by carboxymethyl high-performance membrane chromatography. / Dosio, Franco; Arpicco, Silvia; Canevari, Silvana; Figini, Mariangela; Gastaldi, Daniela.
In: Journal of Chromatography A, Vol. 830, No. 2, 15.01.1999, p. 329-335.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Single-step purification of immunotoxins containing a high ionic charge ribosome inactivating protein clavin by carboxymethyl high-performance membrane chromatography
AU - Dosio, Franco
AU - Arpicco, Silvia
AU - Canevari, Silvana
AU - Figini, Mariangela
AU - Gastaldi, Daniela
PY - 1999/1/15
Y1 - 1999/1/15
N2 - High-performance membrane chromatography (HPMC) and HPLC hydroxyapatite chromatography were compared for their efficiency in purifying immunotoxins (ITs) containing the ribosome-inactivating protein clavin, which is characterized by a high anionic charge and a low molecular mass. Both methods efficiently removed unreacted clavin from the conjugate crude mixture, but only the cation-exchange HPMC allowed efficient single-step separation of the unreacted monoclonal antibody (mAb) from ITs obtained by different coupling procedures. Copyright (C) 1999 Elsevier Science B.V.
AB - High-performance membrane chromatography (HPMC) and HPLC hydroxyapatite chromatography were compared for their efficiency in purifying immunotoxins (ITs) containing the ribosome-inactivating protein clavin, which is characterized by a high anionic charge and a low molecular mass. Both methods efficiently removed unreacted clavin from the conjugate crude mixture, but only the cation-exchange HPMC allowed efficient single-step separation of the unreacted monoclonal antibody (mAb) from ITs obtained by different coupling procedures. Copyright (C) 1999 Elsevier Science B.V.
KW - Clavin
KW - Immunotoxins
KW - Membranes
KW - Monoclonal antibodies
KW - Proteins
KW - Toxins
UR - http://www.scopus.com/inward/record.url?scp=0032925701&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032925701&partnerID=8YFLogxK
U2 - 10.1016/S0021-9673(98)00933-9
DO - 10.1016/S0021-9673(98)00933-9
M3 - Article
C2 - 10048197
AN - SCOPUS:0032925701
VL - 830
SP - 329
EP - 335
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
IS - 2
ER -