Single-step purification of immunotoxins containing a high ionic charge ribosome inactivating protein clavin by carboxymethyl high-performance membrane chromatography

Franco Dosio, Silvia Arpicco, Silvana Canevari, Mariangela Figini, Daniela Gastaldi

Research output: Contribution to journalArticle

Abstract

High-performance membrane chromatography (HPMC) and HPLC hydroxyapatite chromatography were compared for their efficiency in purifying immunotoxins (ITs) containing the ribosome-inactivating protein clavin, which is characterized by a high anionic charge and a low molecular mass. Both methods efficiently removed unreacted clavin from the conjugate crude mixture, but only the cation-exchange HPMC allowed efficient single-step separation of the unreacted monoclonal antibody (mAb) from ITs obtained by different coupling procedures. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)329-335
Number of pages7
JournalJournal of Chromatography A
Volume830
Issue number2
DOIs
Publication statusPublished - Jan 15 1999

Fingerprint

Ribosome Inactivating Proteins
Immunotoxins
Chromatography
Purification
Membranes
Molecular mass
Durapatite
Cations
Ion exchange
High Pressure Liquid Chromatography
Monoclonal Antibodies

Keywords

  • Clavin
  • Immunotoxins
  • Membranes
  • Monoclonal antibodies
  • Proteins
  • Toxins

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Single-step purification of immunotoxins containing a high ionic charge ribosome inactivating protein clavin by carboxymethyl high-performance membrane chromatography. / Dosio, Franco; Arpicco, Silvia; Canevari, Silvana; Figini, Mariangela; Gastaldi, Daniela.

In: Journal of Chromatography A, Vol. 830, No. 2, 15.01.1999, p. 329-335.

Research output: Contribution to journalArticle

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