Single-step purification of immunotoxins containing a high ionic charge ribosome inactivating protein clavin by carboxymethyl high-performance membrane chromatography

Franco Dosio; Silvia Arpicco; Silvana Canevari; Mariangela Figini; Daniela Gastaldi

doi:10.1016/S0021-9673(98)00933-9

Single-step purification of immunotoxins containing a high ionic charge ribosome inactivating protein clavin by carboxymethyl high-performance membrane chromatography

Franco Dosio, Silvia Arpicco, Silvana Canevari, Mariangela Figini, Daniela Gastaldi

Fondazione IRCCS Istituto Nazionale dei Tumori

Research output: Contribution to journal › Article › peer-review

Abstract

High-performance membrane chromatography (HPMC) and HPLC hydroxyapatite chromatography were compared for their efficiency in purifying immunotoxins (ITs) containing the ribosome-inactivating protein clavin, which is characterized by a high anionic charge and a low molecular mass. Both methods efficiently removed unreacted clavin from the conjugate crude mixture, but only the cation-exchange HPMC allowed efficient single-step separation of the unreacted monoclonal antibody (mAb) from ITs obtained by different coupling procedures. Copyright (C) 1999 Elsevier Science B.V.

Original language	English
Pages (from-to)	329-335
Number of pages	7
Journal	Journal of Chromatography A
Volume	830
Issue number	2
DOIs	https://doi.org/10.1016/S0021-9673(98)00933-9
Publication status	Published - Jan 15 1999

Keywords

Clavin
Immunotoxins
Membranes
Monoclonal antibodies
Proteins
Toxins

ASJC Scopus subject areas

Analytical Chemistry

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10.1016/S0021-9673(98)00933-9

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title = "Single-step purification of immunotoxins containing a high ionic charge ribosome inactivating protein clavin by carboxymethyl high-performance membrane chromatography",

abstract = "High-performance membrane chromatography (HPMC) and HPLC hydroxyapatite chromatography were compared for their efficiency in purifying immunotoxins (ITs) containing the ribosome-inactivating protein clavin, which is characterized by a high anionic charge and a low molecular mass. Both methods efficiently removed unreacted clavin from the conjugate crude mixture, but only the cation-exchange HPMC allowed efficient single-step separation of the unreacted monoclonal antibody (mAb) from ITs obtained by different coupling procedures. Copyright (C) 1999 Elsevier Science B.V.",

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T1 - Single-step purification of immunotoxins containing a high ionic charge ribosome inactivating protein clavin by carboxymethyl high-performance membrane chromatography

AU - Dosio, Franco

AU - Arpicco, Silvia

AU - Canevari, Silvana

AU - Figini, Mariangela

AU - Gastaldi, Daniela

PY - 1999/1/15

Y1 - 1999/1/15

N2 - High-performance membrane chromatography (HPMC) and HPLC hydroxyapatite chromatography were compared for their efficiency in purifying immunotoxins (ITs) containing the ribosome-inactivating protein clavin, which is characterized by a high anionic charge and a low molecular mass. Both methods efficiently removed unreacted clavin from the conjugate crude mixture, but only the cation-exchange HPMC allowed efficient single-step separation of the unreacted monoclonal antibody (mAb) from ITs obtained by different coupling procedures. Copyright (C) 1999 Elsevier Science B.V.

AB - High-performance membrane chromatography (HPMC) and HPLC hydroxyapatite chromatography were compared for their efficiency in purifying immunotoxins (ITs) containing the ribosome-inactivating protein clavin, which is characterized by a high anionic charge and a low molecular mass. Both methods efficiently removed unreacted clavin from the conjugate crude mixture, but only the cation-exchange HPMC allowed efficient single-step separation of the unreacted monoclonal antibody (mAb) from ITs obtained by different coupling procedures. Copyright (C) 1999 Elsevier Science B.V.

KW - Clavin

KW - Immunotoxins

KW - Membranes

KW - Monoclonal antibodies

KW - Proteins

KW - Toxins

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JO - Journal of Chromatography A

JF - Journal of Chromatography A

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Single-step purification of immunotoxins containing a high ionic charge ribosome inactivating protein clavin by carboxymethyl high-performance membrane chromatography

Abstract

Keywords

ASJC Scopus subject areas

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