Abstract
High-performance membrane chromatography (HPMC) and HPLC hydroxyapatite chromatography were compared for their efficiency in purifying immunotoxins (ITs) containing the ribosome-inactivating protein clavin, which is characterized by a high anionic charge and a low molecular mass. Both methods efficiently removed unreacted clavin from the conjugate crude mixture, but only the cation-exchange HPMC allowed efficient single-step separation of the unreacted monoclonal antibody (mAb) from ITs obtained by different coupling procedures. Copyright (C) 1999 Elsevier Science B.V.
Original language | English |
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Pages (from-to) | 329-335 |
Number of pages | 7 |
Journal | Journal of Chromatography A |
Volume | 830 |
Issue number | 2 |
DOIs | |
Publication status | Published - Jan 15 1999 |
Keywords
- Clavin
- Immunotoxins
- Membranes
- Monoclonal antibodies
- Proteins
- Toxins
ASJC Scopus subject areas
- Analytical Chemistry