Single-target high-throughput transcription analyses reveal high levels of alternative splicing present in the FPPS/GGPPS from Plasmodium falciparum

Heloisa B. Gabriel, Mauro F. De Azevedo, Giuseppe Palmisano, Gerhard Wunderlich, Emília A. Kimura, Alejandro M. Katzin, João M P Alves

Research output: Contribution to journalArticle

Abstract

Malaria is a tropical disease with significant morbidity and mortality. A better understanding of the metabolism of its most important etiological agent, Plasmodium falciparum, is paramount to the development of better treatment and other mitigation measures. Farnesyldiphosphate synthase/geranylgeranyldiphosphate synthase (FPPS/GGPPS) is a key enzyme in the synthesis of isoprenic chains present in many essential structures. In P. falciparum, as well as a handful of other organisms, FPPS/GGPPS has been shown to be a bifunctional enzyme. By genetic tagging and microscopy, we observed a changing localization of FPPS/GGPPS in blood stage parasites. Given the great importance of alternative splicing and other transcriptional phenomena in gene regulation and the generation of protein diversity, we have investigated the processing of the FPPS/GGPPS transcript in P. falciparum by high-throughput sequencing methods in four time-points along the intraerythrocytic cycle of P. falciparum. We have identified levels of transcript diversity an order of magnitude higher than previously observed in this organism, as well as a few stage-specific splicing events. Our data suggest that alternative splicing in P. falciparum is an important feature for gene regulation and the generation of protein diversity.

Original languageEnglish
Article number18429
JournalScientific Reports
Volume5
DOIs
Publication statusPublished - Dec 21 2015

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Alternative Splicing
Plasmodium falciparum
Enzymes
Genes
Malaria
Microscopy
Parasites
Proteins
Morbidity
Mortality

ASJC Scopus subject areas

  • General

Cite this

Gabriel, H. B., De Azevedo, M. F., Palmisano, G., Wunderlich, G., Kimura, E. A., Katzin, A. M., & Alves, J. M. P. (2015). Single-target high-throughput transcription analyses reveal high levels of alternative splicing present in the FPPS/GGPPS from Plasmodium falciparum. Scientific Reports, 5, [18429]. https://doi.org/10.1038/srep18429

Single-target high-throughput transcription analyses reveal high levels of alternative splicing present in the FPPS/GGPPS from Plasmodium falciparum. / Gabriel, Heloisa B.; De Azevedo, Mauro F.; Palmisano, Giuseppe; Wunderlich, Gerhard; Kimura, Emília A.; Katzin, Alejandro M.; Alves, João M P.

In: Scientific Reports, Vol. 5, 18429, 21.12.2015.

Research output: Contribution to journalArticle

Gabriel, Heloisa B. ; De Azevedo, Mauro F. ; Palmisano, Giuseppe ; Wunderlich, Gerhard ; Kimura, Emília A. ; Katzin, Alejandro M. ; Alves, João M P. / Single-target high-throughput transcription analyses reveal high levels of alternative splicing present in the FPPS/GGPPS from Plasmodium falciparum. In: Scientific Reports. 2015 ; Vol. 5.
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