Single tube genotyping of GSTM1, GSTT1 and TP53 polymorphisms by multiplex PCR

Fabio Bottari, Stefano Landi, Federica Gemignani

Research output: Contribution to journalArticle

Abstract

Glutathione S-transferases (GST) are enzymes involved in the conjugation of a number of human carcinogens, while p53 tumour suppressor gene is the most frequently mutated gene identified till now in human neoplasias. Typically, GSTM1 and GSTT1 genotyping are performed together, with several different protocol described and sometimes with the risk of misclassification due to "false negative", depending on the internal positive control employed. Here, we report a modification of the classical multiplex polymerase chain reaction (PCR) method, allowing the genotyping of GSTM1, GSTT1, together with a polymorphism within the intron 3 of TP53 tumour suppressor gene (a 16 base pairs (bp) duplication) in a single tube, with an appropriate internal positive control. To test the applicability of the method, the frequencies of the deleted alleles of GSTM1 and GSTT1 (null genotypes), and the 16 bp duplication of TP53 gene were assayed in a series of Caucasian DNA samples.

Original languageEnglish
Pages (from-to)396-399
Number of pages4
JournalDNA Sequence - Journal of DNA Sequencing and Mapping
Volume17
Issue number5
DOIs
Publication statusPublished - Oct 2006

Keywords

  • Genotyping
  • GSTM1
  • GSTT1
  • Multiplex PCR
  • TP53

ASJC Scopus subject areas

  • Genetics
  • Biochemistry
  • Endocrinology
  • Molecular Biology

Fingerprint Dive into the research topics of 'Single tube genotyping of GSTM1, GSTT1 and TP53 polymorphisms by multiplex PCR'. Together they form a unique fingerprint.

  • Cite this