Abstract
We report the development of a single-tube assay for rapid genotyping of the two mutations C282Y and H63D of the HFE gene, commonly detected by PCR-RFLP and responsible for 80% of cases of hereditary hemochromatosis. The method, named T melting curve analysis, discriminates between amplification products by their melting temperatures.
Original language | English |
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Pages (from-to) | 990-991 |
Number of pages | 2 |
Journal | Haematologica |
Volume | 85 |
Issue number | 9 |
Publication status | Published - 2000 |
Keywords
- Hemochromatosis
- HFE
- Melting temperature
- Mutation detection
ASJC Scopus subject areas
- Hematology